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Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using Spartan Polyclonal antibody (24850-1-AP)

    WB analysis of human placenta using 24850-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 24850-1-AP (Spartan antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using Spartan Polyclonal antibody (24850-1-AP)

    IP experiment of HEK-293 using 24850-1-AP

    IP result of anti-Spartan (IP:24850-1-AP, 4ug; Detection:24850-1-AP 1:500) with HEK-293 cells lysate 1800 ug.

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Spartan Polyclonal antibody

Spartan Polyclonal Antibody for WB, IP, ELISA
Cat No. 24850-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Synonyms

C1orf124, DVC1, SPRTN, PRO4323, DDDL1880

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman placenta tissue
Positive IP detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24850-1-AP targets Spartan in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Spartan fusion protein Ag20586 相同性解析による交差性が予測される生物種
Full Name DNA-dependent metalloprotease SPRTN
Calculated molecular weight 489 aa, 55 kDa
Observed molecular weight55 kDa, 65 kDa
GenBank accession numberBC015740
Gene Symbol Spartan
Gene ID (NCBI) 83932
RRIDAB_3669456
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H040
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Spartan (Protein with SprT-like domain at the N terminus), also known as DVC1 or C1orf124, is a replication-coupled DNA-dependent metalloprotease that cleaves proteins cross-linked to DNA to promote DPC repair (PMID: 38600235). Spartan protease activity is stimulated by DNA binding, while posttranslational modification of Spartan governs both its protease activity and recruitment to the DPC on chromatin (PMID: 27871366). The regulation of Spartan includes a ubiquitin switch (where deubiquitination activates its proteolytic activity), and acetylation and phosphorylation switches (which facilitate DNA binding). The calculated molecular weight of Spartan is 55 kDa, while the 65-kDa band could be due to posttranslational modification (PMID: 33567341, 31316063).

Protocols

Product Specific Protocols
WB protocol for Spartan antibody 24850-1-APDownload protocol
IP protocol for Spartan antibody 24850-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols