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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SPTA1 Polyclonal antibody (55227-1-AP)

    WB analysis using 55227-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55227-1-AP (SPTA1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of mouse lung tissue using SPTA1 Polyclonal antibody (55227-1-AP)

    IF Staining of mouse lung using 55227-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse lung tissue using SPTA1 antibody (55227-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of K-562 cells using SPTA1 Polyclonal antibody (55227-1-AP)

    FC experiment of K-562 using 55227-1-AP

    1x10^6 K-562 cells were intracellularly stained with 0.4 ug SPTA1 Polyclonal antibody (55227-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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SPTA1 Polyclonal antibody

SPTA1 Polyclonal Antibody for WB, IF-P, FC (Intra), ELISA
Cat No. 55227-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IF-P, FC (Intra), ELISA

Synonyms

EL2, Alpha I spectrin

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inrat red blood cells, human blood
Positive IF-P detected inmouse lung tissue
Positive FC (Intra) detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55227-1-AP targets SPTA1 in WB, IF-P, FC (Intra), ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name spectrin, alpha, erythrocytic 1 (elliptocytosis 2)
Calculated molecular weight 280 kDa
Observed molecular weight 280 kDa
GenBank accession numberNM_003126
Gene Symbol SPTA1
Gene ID (NCBI) 6708
RRIDAB_3086436
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP02549
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Alpha 1 Spectrin also known as SPTA1, EL2, belongs to the spectrin family. Spectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane. It associates with band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane. Mutations in SPTA1 result in a variety of hereditary red blood cell disorders, including elliptocytosis-2, pyropoikilocytosis, and spherocytosis, type 3(PMID: 1541680, 8941647).

Protocols

Product Specific Protocols
WB protocol for SPTA1 antibody 55227-1-APDownload protocol
IF protocol for SPTA1 antibody 55227-1-APDownload protocol
FC protocol for SPTA1 antibody 55227-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols