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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SPTB Polyclonal antibody

SPTB Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 26936-1-AP

Synonyms

Spectrin beta chain, erythrocytic, Beta-I spectrin, Beta I spectrin

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SPTB Polyclonal antibody (26936-1-AP)

    WB analysis using 26936-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26936-1-AP (SPTB antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using SPTB Polyclonal antibody (26936-1-AP)

    IF Staining of U2OS using 26936-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using SPTB antibody (26936-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inK-562 cells, mouse heart tissue
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26936-1-AP targets SPTB in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SPTB fusion protein Ag25614 相同性解析による交差性が予測される生物種
Full Name spectrin, beta, erythrocytic
Observed molecular weight 246 kDa
GenBank accession numberBC136284
Gene symbol SPTB
Gene ID (NCBI) 6710
RRIDAB_3669575
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Beta I spectrin also known as SPTB, SPTB1, belongs to the spectrin family. Spectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane. It associates with band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane. Mutations in SPTB have been associated with Elliptocytosis 3, spherocytosis type 2, and neonatal hemolytic anemia(PMID: 8018926, 8102379, 9005995).

Protocols

Product Specific Protocols
WB protocol for SPTB antibody 26936-1-APDownload protocol
IF protocol for SPTB antibody 26936-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols