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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SPTBN1 Monoclonal antibody (67978-1-Ig)

    WB analysis using 67978-1-Ig (same clone as 67978-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67978-1-Ig (SPTBN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67978-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse cerebellum tissue using SPTBN1 Monoclonal antibody (67978-1-Ig)

    WB analysis of mouse cerebellum using 67978-1-Ig (same clone as 67978-1-PBS)

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 67978-1-Ig (SPTBN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67978-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SPTBN1 Monoclonal antibody (67978-1-Ig)

    IHC staining of human liver cancer using 67978-1-Ig (same clone as 67978-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67978-1-Ig (SPTBN1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67978-1-PBS in a different storage buffer formulation.

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SPTBN1 Monoclonal antibody, PBS Only

SPTBN1 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67978-1-PBS
Clone No.1E1D1

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, rabbit, pig

Applications

WB, IHC, Indirect ELISA

Synonyms

Beta II spectrin, betaSpII, ELF, Fodrin beta chain, Spectrin beta chain, brain 1, SPTB2, SPTBN1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67978-1-PBS targets SPTBN1 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.

Tested Reactivity Human, mouse, rat, rabbit, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SPTBN1 fusion protein Ag31713 相同性解析による交差性が予測される生物種
Full Name spectrin, beta, non-erythrocytic 1
Calculated molecular weight 2364 aa, 275 kDa
Observed molecular weight250-275 kDa
GenBank accession numberBC137282
Gene Symbol SPTBN1
Gene ID (NCBI) 6711
RRIDAB_2918727
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ01082
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

SPTBN1 is the non-erythrocytic form of β-spectrin or β-fodrin. Spectrins are members of the superfamily of F-actin cross linking proteins that are important as scaffolding proteins for protein sorting, cell adhesion, and migration. Reduced expression of SPTBN1 has been found to correlate with shorter survival of patients with hepatocellular cancer, pancreatic cancer and other malignancies of the gastrointestinal tract, suggesting tumor suppression ability of this protein.