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SPTBN2 Polyclonal antibody
SPTBN2 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 55107-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, human brain tissue, mouse brain, rat brain |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 5 publications below |
| IF | See 1 publications below |
Product Information
55107-1-AP targets SPTBN2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, drosophila |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | spectrin, beta, non-erythrocytic 2 |
| Calculated molecular weight | 271 kDa |
| Observed molecular weight | 270 kDa |
| GenBank accession number | NM_006946 |
| Gene symbol | SPTBN2 |
| Gene ID (NCBI) | 6712 |
| RRID | AB_10804779 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SPTBN2, also named as KIAA0302, belongs to the spectrin family. It probably plays an important role in neuronal membrane skeleton. Defects in SPTBN2 are the cause of spinocerebellar ataxia type 5 (SCA5). The antibody is specific to SPTBN2.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SPTBN2 antibody 55107-1-AP | Download protocol |
| IHC protocol for SPTBN2 antibody 55107-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Biol Chem β-Spectrin regulates the Hippo signaling pathway and modulates the basal actin network.
| ||
Front Mol Neurosci Analyzing the mechanisms that facilitate the subtype-specific assembly of γ-aminobutyric acid type A receptors | ||
Cerebellum Nitric Oxide Critically Regulates Purkinje Neuron Dendritic Development Through a Metabotropic Glutamate Receptor Type 1-Mediated Mechanism. | ||
Dis Markers SPTBN2 Promotes the Progression of Thyroid Cancer by Accelerating G1/S Transition and Inhibiting Apoptosis | ||
Exp Ther Med Decreased SPTBN2 expression regulated by the ceRNA network is associated with poor prognosis and immune infiltration in low‑grade glioma |
