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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SPTBN4 Polyclonal antibody (31713-1-AP)

    WB analysis using 31713-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31713-1-AP (SPTBN4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using SPTBN4 Polyclonal antibody (31713-1-AP)

    IF Staining of SH-SY5Y using 31713-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using SPTBN4 antibody (31713-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

SPTBN4 Polyclonal antibody

SPTBN4 Polyclonal Antibody for WB, ELISA
Cat No. 31713-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

SPTBN3, KIAA1642, Beta IV spectrin

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inmouse brain tissue, mouse cerebellum tissue, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31713-1-AP targets SPTBN4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SPTBN4 fusion protein Ag36430 相同性解析による交差性が予測される生物種
Full Name spectrin, beta, non-erythrocytic 4
Calculated molecular weight288kDa
Observed molecular weight 250, 140 kDa
GenBank accession numberNM_020971
Gene Symbol SPTBN4
Gene ID (NCBI) 57731
RRIDAB_3670092
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ9H254
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SPTBN4 also known as beta-IV spectrin, encodes a nonerythrocytic member of the beta-spectrin protein family that is expressed in the brain, peripheral nervous system, pancreas, and skeletal muscle. Spectrins are rod-shaped proteins that were originally identified as part of the lattice-like cytoskeleton under the erythrocyte membrane(PMID: 11086001).

Protocols

Product Specific Protocols
WB protocol for SPTBN4 antibody 31713-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols