Validation Data Gallery
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Tested Applications
| Positive WB detected in | A549 cells, mouse brain tissue, HEK-293 cells, HT-29 cells, HepG2 cells, NIH/3T3 cells |
| Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 16 publications below |
| IHC | See 1 publications below |
| IF | See 2 publications below |
| FC | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
51012-2-AP targets SPTLC2 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | serine palmitoyltransferase, long chain base subunit 2 |
| Calculated molecular weight | 63 kDa |
| Observed molecular weight | 55-65 kDa |
| GenBank accession number | BC005123 |
| Gene Symbol | SPTLC2 |
| Gene ID (NCBI) | 9517 |
| RRID | AB_2195870 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O15270 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SPTLC2 antibody 51012-2-AP | Download protocol |
| IHC protocol for SPTLC2 antibody 51012-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Gastroenterology IRE1A Stimulates Hepatocyte-Derived Extracellular Vesicles That Promote Inflammation in Mice With Steatohepatitis. | ||
Nat Microbiol Sphingolipid biosynthesis induces a conformational change in the murine norovirus receptor and facilitates viral infection. | ||
Mol Cell Proteomics Quantitative proteomics reveals that only a subset of the endoplasmic reticulum contributes to the phagosome. | ||
J Lipid Res ORMDL MISLOCALIZATION BY IMPAIRED AUTOPHAGY IN NIEMANN-PICK TYPE C DISEASE LEADS TO INCREASED DE NOVO SPHINGOLIPID BIOSYNTHESIS | ||
Cell Death Dis Sphingolipid de novo biosynthesis is essential for intestine cell survival and barrier function.
| ||
J Biol Chem Liver-specific deficiency of serine palmitoyltransferase subunit 2 decreases plasma sphingomyelin and increases apolipoprotein E levels. |