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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SPTLC3 Polyclonal antibody

SPTLC3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16600-1-AP

Synonyms

Long chain base biosynthesis protein 3, Long chain base biosynthesis protein 2b, LCB2b, LCB 3, EC:2.3.1.50

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SPTLC3 Polyclonal antibody (16600-1-AP)

    WB analysis using 16600-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16600-1-AP (SPTLC3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using SPTLC3 Polyclonal antibody (16600-1-AP)

    IHC staining of human ovary cancer using 16600-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 16600-1-AP (SPTLC3 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SPTLC3 Polyclonal antibody (16600-1-AP)

    IF Staining of HepG2 using 16600-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SPTLC3 antibody (16600-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHepG2 cells, mouse liver tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

16600-1-AP targets SPTLC3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SPTLC3 fusion protein Ag9760 相同性解析による交差性が予測される生物種
Full Name serine palmitoyltransferase, long chain base subunit 3
Calculated molecular weight 175 aa, 20 kDa
Observed molecular weight62 kDa
GenBank accession numberBC020656
Gene symbol SPTLC3
Gene ID (NCBI) 55304
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Serine-palmitoyltransferase (SPT) is a pyridoxal 5‐phosphate (PLP)-dependent α‐oxoaminotransferase that consists of three core subunits-SPTLC1, SPTLC2, and SPTLC3-that share a mutual homology. The amino acid sequences between SPTLC1 and SPTLC2 (or SPTLC3) show a mutual similarity of about 20%, whereas the sequences for SPTLC2 and SPTLC3 show a significantly higher identity of 68% (with 84% similarity) on the amino acid level. SPTLC3 is not or only little expressed in tissues that are functionally connected to erythropoiesis or immune response like peripheral blood cells, bone marrow, and spleen. Instead, the lack of SPTLC3 expression seems to be compensated by increased expression of SPTLC2 in these tissues. (PMID: 17331073, PMID: 17023427)

Protocols

Product Specific Protocols
WB protocol for SPTLC3 antibody 16600-1-APDownload protocol
IHC protocol for SPTLC3 antibody 16600-1-APDownload protocol
IF protocol for SPTLC3 antibody 16600-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Am J Cancer Res

TRIM8 inhibits breast cancer proliferation by regulating estrogen signaling.

Authors - Zelin Tian
View Article