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  • KD/KO Validated

SQLE Monoclonal antibody

SQLE Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Rat, pig and More (1)

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1C9A2

Cat no : 67206-1-Ig

Synonyms

ERG1, FLJ30795, SE, SQLE, squalene epoxidase, Squalene monooxygenase


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Tested Applications

Positive WB detected inA549 cells, HEK-293 cells, HepG2 cells, HSC-T6 cells, L02 cells
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67206-1-Ig targets SQLE in WB, IF/ICC, ELISA applications and shows reactivity with Human, Rat, pig samples.

Tested Reactivity Human, Rat, pig
Cited Reactivitymouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen SQLE fusion protein Ag3266 相同性解析による交差性が予測される生物種
Full Name squalene epoxidase
Calculated molecular weight 574 aa, 64 kDa
Observed molecular weight 50-64 kDa
GenBank accession numberBC017033
Gene symbol SQLE
Gene ID (NCBI) 6713
RRIDAB_2882499
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SQLE, also named as ERG1, SE and SM, belongs to the squalene monooxygenase family. It catalyzes the first oxygenation step in cholesterol synthesis, acting on squalene before cyclization into the basic steroid structure. SQLE may serve as a flux-controlling enzyme beyond 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGR, considered as rate limiting). It is also posttranslationally regulated by cholesterol-dependent proteasomal degradation. SQLE is subject to feedback regulation via cholesterol-induced degradation, which depends on its lipid-sensing N terminal regulatory domain. Truncation of SQLE occurs during its endoplasmic reticulum-associated degradation and requires the proteasome, which partially degrades the SQLE N-terminus and eliminates cholesterol-sensing elements within this region. The MW of SQLE is about 50-64 kDa. (PMID:21356516, PMID: 28972164)

Protocols

Product Specific Protocols
WB protocol for SQLE antibody 67206-1-IgDownload protocol
IF protocol for SQLE antibody 67206-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Adv Sci (Weinh)

Targeting Squalene Epoxidase Confers Metabolic Vulnerability and Overcomes Chemoresistance in HNSCC

Authors - Xinyuan Zhao
mouseIF

Cell Signal

Knockdown of SQLE promotes CD8+ T cell infiltration in the tumor microenvironment

Authors - Jun Wu
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Boyan (Verified Customer) (11-24-2022)

Detected a band around the expected molecular weight, but with some other background bands.

  • Applications: Western Blot