Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, HEK-293 cells, HepG2 cells, HSC-T6 cells, L02 cells |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
| IF | See 1 publications below |
Product Information
67206-1-Ig targets SQLE in WB, IF/ICC, ELISA applications and shows reactivity with Human, Rat, pig samples.
| Tested Reactivity | Human, Rat, pig |
| Cited Reactivity | mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | SQLE fusion protein Ag3266 相同性解析による交差性が予測される生物種 |
| Full Name | squalene epoxidase |
| Calculated molecular weight | 574 aa, 64 kDa |
| Observed molecular weight | 50-64 kDa |
| GenBank accession number | BC017033 |
| Gene Symbol | SQLE |
| Gene ID (NCBI) | 6713 |
| RRID | AB_2882499 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q14534 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SQLE, also named as ERG1, SE and SM, belongs to the squalene monooxygenase family. It catalyzes the first oxygenation step in cholesterol synthesis, acting on squalene before cyclization into the basic steroid structure. SQLE may serve as a flux-controlling enzyme beyond 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGR, considered as rate limiting). It is also posttranslationally regulated by cholesterol-dependent proteasomal degradation. SQLE is subject to feedback regulation via cholesterol-induced degradation, which depends on its lipid-sensing N terminal regulatory domain. Truncation of SQLE occurs during its endoplasmic reticulum-associated degradation and requires the proteasome, which partially degrades the SQLE N-terminus and eliminates cholesterol-sensing elements within this region. The MW of SQLE is about 50-64 kDa. (PMID:21356516, PMID: 28972164)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SQLE antibody 67206-1-Ig | Download protocol |
| IF protocol for SQLE antibody 67206-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Targeting Squalene Epoxidase Confers Metabolic Vulnerability and Overcomes Chemoresistance in HNSCC | ||
Cell Signal Knockdown of SQLE promotes CD8+ T cell infiltration in the tumor microenvironment
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