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SQRDL Monoclonal antibody

SQRDL Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

1B4E3

Cat no : 68459-1-Ig

Synonyms

Sulfide dehydrogenase-like, SQOR, EC:1.8.5.8, CGI-44, CGI 44

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SQRDL Monoclonal antibody (68459-1-Ig)

    WB analysis using 68459-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68459-1-Ig (SQRDL antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using SQRDL Monoclonal antibody (68459-1-Ig)

    WB analysis of A549 using 68459-1-Ig

    WB result of SQRDL antibody (68459-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SQRDL transfected A549 cells.

  • Immunoprecipitation (IP) experiment of HeLa cells using SQRDL Monoclonal antibody (68459-1-Ig)

    IP experiment of HeLa using 68459-1-Ig

    IP result of anti-SQRDL (IP:68459-1-Ig, 4ug; Detection:68459-1-Ig 1:3000) with HeLa cells lysate 1560 ug.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using SQRDL Monoclonal antibody (68459-1-Ig)

    IHC staining of human skin cancer using 68459-1-Ig

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 68459-1-Ig (SQRDL antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SQRDL Monoclonal antibody (68459-1-Ig)

    IF Staining of HepG2 using 68459-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SQRDL antibody (68459-1-Ig, Clone: 1B4E3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Tested Applications

Positive WB detected inA549 cells, HeLa cells, HepG2 cells, HT-29 cells, hTERT-RPE1 cells, THP-1 cells, pig liver tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68459-1-Ig targets SQRDL in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen SQRDL fusion protein Ag10762 相同性解析による交差性が予測される生物種
Full Name sulfide quinone reductase-like (yeast)
Calculated molecular weight 450 aa, 50 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC016836
Gene symbol SQRDL
Gene ID (NCBI) 58472
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SQRDL(Sulfide:quinone oxidoreductase, mitochondrial) catalyzes the oxidation of hydrogen sulfide, with the help of a quinone.Although the SQRDL precursor protein displays a molecular weight of 50 kDa,a prominent band at about 46 kDa is detected by western blot. This discrepancy reflects the cleavage of an N-terminal targeting sequence of 4 kDa during the import of the protein into the mitochondria(PMID:22067608).SQRDL is one of the enzymes involved in H2S signaling in a discrete population of neurons, oligodendrocytes, and endothelial cells.

Protocols

Product Specific Protocols
WB protocol for SQRDL antibody 68459-1-IgDownload protocol
IHC protocol for SQRDL antibody 68459-1-IgDownload protocol
IF protocol for SQRDL antibody 68459-1-IgDownload protocol
IP protocol for SQRDL antibody 68459-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols