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- KD/KO Validated
FDFT1 Polyclonal antibody
FDFT1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 13128-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, T-47D cells |
| Positive IP detected in | COLO 320 cells |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | L02 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 5 publications below |
| WB | See 17 publications below |
| IHC | See 3 publications below |
Product Information
13128-1-AP targets FDFT1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | FDFT1 fusion protein Ag3743 相同性解析による交差性が予測される生物種 |
| Full Name | farnesyl-diphosphate farnesyltransferase 1 |
| Calculated molecular weight | 417 aa, 48 kDa |
| Observed molecular weight | 48 kDa |
| GenBank accession number | BC029641 |
| Gene symbol | FDFT1 |
| Gene ID (NCBI) | 2222 |
| RRID | AB_2294094 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Farnesyl-diphosphate farnesyltransferase 1 (FDFT1), also known as SQS, is a gene that encodes the membrane-associated enzyme squalene synthase, which is the first specific enzyme in cholesterol biosynthesis. FDFT1 is highly expressed in liver, lung, prostate, breast, ovary, bladder, cervix, thyroid, and esophageal cancers, while in colorectal, colon, testicular, uterine, pancreas, and kidney tumors, its expression is downregulated (PMID: 35093030). FDFT1 is a key downstream target of the fasting response and may be involved in CRC cell glucose metabolism (PMID: 32313017).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FDFT1 antibody 13128-1-AP | Download protocol |
| IHC protocol for FDFT1 antibody 13128-1-AP | Download protocol |
| IF protocol for FDFT1 antibody 13128-1-AP | Download protocol |
| IP protocol for FDFT1 antibody 13128-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Metab Functional Genomics Identifies Metabolic Vulnerabilities in Pancreatic Cancer.
| ||
Adv Sci (Weinh) Endothelial Cells Mediated by STING Regulate Oligodendrogenesis and Myelination During Brain Development | ||
Neuron Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory. | ||
Cancer Res Caffeine supplementation and FOXM1 inhibition enhance the antitumor effect of statins in neuroblastoma
| ||
Phytomedicine Hibiscus syriacus L. cultivated in callus culture exerts cytotoxicity in colorectal cancer via Notch signaling-mediated cholesterol biosynthesis suppression. |
