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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SR140 Polyclonal antibody

SR140 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21399-1-AP

Synonyms

U2SURP, U2-associated protein SR140, U2 snRNP-associated SURP motif-containing protein, KIAA0332, 140 kDa Ser/Arg-rich domain protein

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse heart tissue using SR140 Polyclonal antibody (21399-1-AP)

    WB analysis of mouse heart using 21399-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 21399-1-AP (SR140 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using SR140 Polyclonal antibody (21399-1-AP)

    WB analysis of HeLa using 21399-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21399-1-AP (SR140 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using SR140 Polyclonal antibody (21399-1-AP)

    WB analysis of human brain using 21399-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 21399-1-AP (SR140 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using SR140 Polyclonal antibody (21399-1-AP)

    WB analysis of L02 using 21399-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 21399-1-AP (SR140 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SR140 Polyclonal antibody (21399-1-AP)

    IHC staining of human breast cancer using 21399-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 21399-1-AP (SR140 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SR140 Polyclonal antibody (21399-1-AP)

    IHC staining of human breast cancer using 21399-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 21399-1-AP (SR140 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using SR140 Polyclonal antibody (21399-1-AP)

    IF Staining of HEK-293 using 21399-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using SR140 antibody (21399-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SR140 Polyclonal antibody (21399-1-AP)

    IF Staining of HeLa using 21399-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SR140 antibody (21399-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SR140 Polyclonal antibody (21399-1-AP)

    IF Staining of HepG2 using 21399-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SR140 antibody (21399-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SR140 Polyclonal antibody (21399-1-AP)

    IF Staining of HepG2 using 21399-1-AP

    Immunofluorescent analysis of HepG2 cells, using SR140 antibody 21399-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SR140 Polyclonal antibody (21399-1-AP)

    IF Staining of HeLa using 21399-1-AP

    Immunofluorescent analysis of Hela cells, using SR140 antibody 21399-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

Positive WB detected inmouse heart tissue, HeLa cells, human brain tissue, L02 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, HepG2 cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21399-1-AP targets SR140 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SR140 fusion protein Ag16093 相同性解析による交差性が予測される生物種
Full Name U2-associated SR140 protein
Calculated molecular weight 1029 aa, 118 kDa
Observed molecular weight 120-140 kDa
GenBank accession numberBC111692
Gene symbol SR140
Gene ID (NCBI) 23350
RRIDAB_10863661
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SR140 antibody 21399-1-APDownload protocol
IHC protocol for SR140 antibody 21399-1-APDownload protocol
IF protocol for SR140 antibody 21399-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols