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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SRBD1 Polyclonal antibody

SRBD1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24931-1-AP

Synonyms

S1 RNA binding domain 1, SRBD1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SRBD1 Polyclonal antibody (24931-1-AP)

    WB analysis using 24931-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 24931-1-AP (SRBD1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using SRBD1 Polyclonal antibody (24931-1-AP)

    IP experiment of mouse brain using 24931-1-AP

    IP result of anti-SRBD1 (IP:24931-1-AP, 4ug; Detection:24931-1-AP 1:300) with mouse brain tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human brain tissue using SRBD1 Polyclonal antibody (24931-1-AP)

    IHC staining of human brain using 24931-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24931-1-AP (SRBD1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using SRBD1 Polyclonal antibody (24931-1-AP)

    IHC staining of human brain using 24931-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24931-1-AP (SRBD1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inSH-SY5Y cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24931-1-AP targets SRBD1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SRBD1 fusion protein Ag21661 相同性解析による交差性が予測される生物種
Full Name S1 RNA binding domain 1
Calculated molecular weight 995 aa, 112 kDa
Observed molecular weight 100-120 kDa
GenBank accession numberBC032538
Gene symbol SRBD1
Gene ID (NCBI) 55133
RRIDAB_2879805
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

S1 RNA-binding domain-containing protein 1 (SRBD1) is a 995 amino acid protein, which contains one S1 motif domain. SRBD1 is a susceptibility gene for normal tension glaucoma (NTG) and may be involved in cell growth inhibition and apoptosis. Two isoforms of SRBD1 exist due to alternative splicing events, and the gene which encodes SRBD1 maps to human chromosome 2p21.

Protocols

Product Specific Protocols
WB protocol for SRBD1 antibody 24931-1-APDownload protocol
IHC protocol for SRBD1 antibody 24931-1-APDownload protocol
IP protocol for SRBD1 antibody 24931-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols