c-SRC Polyclonal antibody

Name: c-SRC Polyclonal antibody
Brand: Proteintech
SKU: 11097-1-AP

c-SRC Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, hamster

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 11097-1-AP

Synonyms

SRC, Proto-oncogene c-Src, pp60c-src, p60-Src, EC:2.7.10.2

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 11097-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11097-1-AP (SRC antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 11097-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 11097-1-AP (SRC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 11097-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 11097-1-AP (SRC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of CHO using 11097-1-AP
CHO cells were subjected to SDS PAGE followed by western blot with 11097-1-AP (SRC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of SH-SY5Y using 11097-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 11097-1-AP (SRC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 11097-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 11097-1-AP (SRC antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 11097-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 11097-1-AP (SRC antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 11097-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 11097-1-AP (SRC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of SH-SY5Y using 11097-1-AP
IP result of anti-c-SRC (IP:11097-1-AP, 5ug; Detection:11097-1-AP 1:1000) with SH-SY5Y cells lysate 2900ug.
IHC staining of human breast cancer using 11097-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11097-1-AP (c-SRC Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 11097-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11097-1-AP (c-SRC Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human brain using 11097-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 11097-1-AP (c-SRC Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human brain using 11097-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 11097-1-AP (c-SRC Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human spleen using 11097-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 11097-1-AP (c-SRC Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human spleen using 11097-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 11097-1-AP (c-SRC Antibody) at dilution of 1:50 (under 40x lens).
IF Staining of A431 using 11097-1-AP
Immunofluorescent analysis of A431 cells using 11097-1-AP (SRC antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

-/ -

Cat No. 11097-1-AP

Size/Concentration

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HUVEC cells, mouse brain tissue, COLO 320 cells, Jurkat cells, A431 cells, CHO cells, SH-SY5Y cells
Positive IP detected in	SH-SY5Y cells
Positive IHC detected in	human breast cancer tissue, human brain tissue, human spleen tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A431 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:100-1:400
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 62 publications below
IHC	See 10 publications below
IF	See 2 publications below
IP	See 4 publications below
CoIP	See 1 publications below

Product Information

11097-1-AP targets c-SRC in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, hamster samples.

Tested Reactivity	human, mouse, rat, hamster
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	c-SRC fusion protein Ag1575 相同性解析による交差性が予測される生物種
Full Name	v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)
Calculated molecular weight	60 kDa
Observed molecular weight	55 kDa, 60 kDa
GenBank accession number	BC011566
Gene symbol	SRC
Gene ID (NCBI)	6714
RRID	AB_2286705
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SRC, also named as SRC1 and p60-Src, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It is a non-receptor protein tyrosine kinase that plays pivotal roles in numerous cellular processes such as proliferation, migration, and transformation. In concert with PTK2B, SRC plays an important role in osteoclastic bone resorption. It promotes energy production in osteoclasts by activating mitochondrial cytochrome C oxidase. SRC can be detected as 60 kDa and 55 kDa (PMID: 15047935, 21461171).

Protocols

Product Specific Protocols
WB protocol for c-SRC antibody 11097-1-AP	Download protocol
IHC protocol for c-SRC antibody 11097-1-AP	Download protocol
IF protocol for c-SRC antibody 11097-1-AP	Download protocol
IP protocol for c-SRC antibody 11097-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Genet Pancreatic cancer risk variant in LINC00673 creates a miR-1231 binding site and interferes with PTPN11 degradation. Authors - Jian Zheng View Article
mouse	WB,IP	Cell Stem Cell Amino acid catabolism regulates hematopoietic stem cell proteostasis via a GCN2-eIF2α axis. Authors - Changzheng Li View Article
human	WB	Sci Adv Platelet P-selectin initiates cross-presentation and dendritic cell differentiation in blood monocytes. Authors - Patrick Han View Article
		J Cell Biol Metalloproteinase MT1-MMP islets act as memory devices for podosome reemergence. Authors - Karim El Azzouzi View Article
human		Cell Death Differ The E3 ubiquitin ligase TRIM7 suppressed hepatocellular carcinoma progression by directly targeting Src protein. Authors - Lihui Zhu View Article
mouse	WB	Haematologica NLRP3 regulates platelet integrin αIIbβ3 outside-in signaling, hemostasis and arterial thrombosis. Authors - Jianlin Qiao View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

c-SRC Polyclonal antibody

c-SRC Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 11097-1-AP

WB analysis of Jurkat using 11097-1-AP

WB analysis of A431 using 11097-1-AP

WB analysis of CHO using 11097-1-AP

WB analysis of SH-SY5Y using 11097-1-AP

WB analysis of mouse brain using 11097-1-AP

WB analysis of COLO 320 using 11097-1-AP

WB analysis of A431 using 11097-1-AP

IP experiment of SH-SY5Y using 11097-1-AP

IHC staining of human breast cancer using 11097-1-AP

IHC staining of human breast cancer using 11097-1-AP

IHC staining of human brain using 11097-1-AP

IHC staining of human brain using 11097-1-AP

IHC staining of human spleen using 11097-1-AP

IHC staining of human spleen using 11097-1-AP

IF Staining of A431 using 11097-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Pancreatic cancer risk variant in LINC00673 creates a miR-1231 binding site and interferes with PTPN11 degradation.

Amino acid catabolism regulates hematopoietic stem cell proteostasis via a GCN2-eIF2α axis.

Platelet P-selectin initiates cross-presentation and dendritic cell differentiation in blood monocytes.

Metalloproteinase MT1-MMP islets act as memory devices for podosome reemergence.

The E3 ubiquitin ligase TRIM7 suppressed hepatocellular carcinoma progression by directly targeting Src protein.

NLRP3 regulates platelet integrin αIIbβ3 outside-in signaling, hemostasis and arterial thrombosis.

Related Products

c-Cbl Polyclonal antibody

CRK Polyclonal antibody

EGFR (N-terminal) Polyclonal antibody

STAT3 Polyclonal antibody

CRK Polyclonal antibody