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  • Featured Product
  • KD/KO Validated

SRD5A1 Monoclonal antibody, PBS Only

SRD5A1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D5D8

Cat no : 66329-1-PBS

Synonyms

S5AR 1, SR type 1, SRD5A1, Steroid 5 alpha reductase 1

Validation Data Gallery

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  • Western Blot (WB) analysis of human testis tissue using SRD5A1 Monoclonal antibody (66329-1-Ig)

    WB analysis of human testis using 66329-1-Ig (same clone as 66329-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 66329-1-Ig (SRD5A1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66329-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SRD5A1 Monoclonal antibody (66329-1-Ig)

    WB analysis using 66329-1-Ig (same clone as 66329-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66329-1-Ig (SRD5A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66329-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using SRD5A1 Monoclonal antibody (66329-1-Ig)

    IHC staining of human liver using 66329-1-Ig (same clone as 66329-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66329-1-Ig (SRD5A1 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66329-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using SRD5A1 Monoclonal antibody (66329-1-Ig)

    IHC staining of human liver using 66329-1-Ig (same clone as 66329-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66329-1-Ig (SRD5A1 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66329-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SRD5A1 Monoclonal antibody (66329-1-Ig)

    IF Staining of HeLa using 66329-1-Ig (same clone as 66329-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using 66329-1-Ig(SRD5A1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66329-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEPG2 cells using SRD5A1 Monoclonal antibody (66329-1-Ig)

    IF Staining of HEPG2 using 66329-1-Ig (same clone as 66329-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HEPG2 cells using SRD5A1 antibody (66329-1-Ig, Clone: 1D5D8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66329-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66329-1-PBS targets SRD5A1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SRD5A1 fusion protein Ag23351 相同性解析による交差性が予測される生物種
Full Name steroid-5-alpha-reductase, alpha polypeptide 1 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1)
Observed molecular weight 32 kDa
GenBank accession numberBC007033
Gene symbol SRD5A1
Gene ID (NCBI) 6715
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Steroid 5-alpha-reductase (SRD5A1)   is a minor component of the reductase activity in prostate although the gene was originally cloned from prostate. On the other hand, SRD5A1 appears to be the predominant isozyme of steroid 5-alpha-reductase in the scalp and elsewhere in the skin.