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SREBF1 Polyclonal antibody

Name: SREBF1 Polyclonal antibody
Brand: Proteintech
SKU: 14088-1-AP
Rating: 5.00 (1 reviews)

SREBF1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

217

Reviews (1)

Cat no : 14088-1-AP

Synonyms

SREBP 1c, SREBP 1, SREBF 1, Processed sterol regulatory element-binding protein 1, Class D basic helix-loop-helix protein 1

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 14088-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14088-1-AP (SREBF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 14088-1-AP
WB result of SREBF1 antibody (14088-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SREBF1 transfected HeLa cells.
WB analysis of HeLa using 14088-1-AP
WB result of SREBF1 antibody (14088-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SREBF1 transfected HeLa cells.
WB analysis using 14088-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14088-1-AP (SREBF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis using 14088-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14088-1-AP (SREBF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis using 14088-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14088-1-AP (SREBF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 14088-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 14088-1-AP (SREBF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of L02 using 14088-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 14088-1-AP (SREBF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of L02 using 14088-1-AP
IP result of anti-SREBF1 (IP:14088-1-AP, 4ug; Detection:14088-1-AP 1:600) with L02 cells lysate 1500ug.
IHC staining of human kidney using 14088-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14088-1-AP (SREBF1 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 14088-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14088-1-AP (SREBF1 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human skeletal muscle using 14088-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 14088-1-AP (SREBF1 Antibody) at dilution of 1:25 (under 10x lens).
IHC staining of human skeletal muscle using 14088-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 14088-1-AP (SREBF1 Antibody) at dilution of 1:25 (under 40x lens).
IF Staining of HeLa using 14088-1-AP
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14088-1-AP (SREBF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 14088-1-AP

Size/Concentration

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, L02 cells, MCF-7 cells, mouse liver tissue, rat liver tissue
Positive IP detected in	L02 cells
Positive IHC detected in	human kidney tissue, human skeletal muscle tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 9 publications below
WB	See 198 publications below
IHC	See 28 publications below
IF	See 24 publications below
IP	See 2 publications below
CoIP	See 1 publications below
ChIP	See 3 publications below

Product Information

14088-1-AP targets SREBF1 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, monkey, chicken, bovine, goat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SREBF1 fusion protein Ag5219 相同性解析による交差性が予測される生物種
Full Name	sterol regulatory element binding transcription factor 1
Calculated molecular weight	1177 aa, 125 kDa
Observed molecular weight	125 kDa, 68 kDa
GenBank accession number	BC063281
Gene symbol	SREBF1
Gene ID (NCBI)	6720
RRID	AB_2255217
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SREBF1, also named as BHLHD1 and SREBP1, contains one basic helix-loop-helix (bHLH) domain and belongs to the SREBP family. It is a transcriptional activator required for lipid homeostasis. The SREBPs are synthesized as precursors anchored to endoplasmic reticulum (ER) membranes and complexed with SCAP. When the cellular cholesterol level is low, SREBP-SCAP complexes move to the Golgi apparatus, where SREBPs undergo a two-step proteolytic processing, leading to the release of the mature form, an N-terminal fragment, i.e, basic helix-loop-helix leucine zipper transcription factor. These factors enter the nucleus where they bind to sterol regulatory elements (SRE) in the promoter regions of a number of genes whose products mediate the synthesis of cholesterol and fatty acids. [PMID: 21698267]. This antibody can recognize the 125 kDa precursor form and the 68 kDa mature form of human SREBF1.

Protocols

Product Specific Protocols
WB protocol for SREBF1 antibody 14088-1-AP	Download protocol
IHC protocol for SREBF1 antibody 14088-1-AP	Download protocol
IF protocol for SREBF1 antibody 14088-1-AP	Download protocol
IP protocol for SREBF1 antibody 14088-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nature A metastasis map of human cancer cell lines. Authors - Xin Jin KO Validated View Article
mouse	IHC	Nature Divergent transcriptional regulation of astrocyte reactivity across disorders. Authors - Joshua E Burda View Article
mouse	IHC	Cell Metab TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis. Authors - Dong Huang View Article
human	WB	Cell Metab CircACC1 Regulates Assembly and Activation of AMPK Complex under Metabolic Stress. Authors - Qidong Li KD Validated View Article
mouse	WB	Nat Commun Senescence-associated 13-HODE production promotes age-related liver steatosis by directly inhibiting catalase activity Authors - Jinjie Duan View Article
human		Hepatology PMID: 29729186 Authors - Huixia Zhang View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

Isha (Verified Customer) (05-18-2022)

Antibody worked perfect at 1:1000 dilution for W.B. and 1:500 for IF. And for chIP 3ug/ml worked best.

Applications: Western Blot, Immunofluorescence, ChIP

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SREBF1 Polyclonal antibody

SREBF1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 14088-1-AP

WB analysis of HeLa using 14088-1-AP

WB analysis of HeLa using 14088-1-AP

WB analysis using 14088-1-AP

WB analysis using 14088-1-AP

WB analysis using 14088-1-AP

WB analysis of HeLa using 14088-1-AP

WB analysis of L02 using 14088-1-AP

IP experiment of L02 using 14088-1-AP

IHC staining of human kidney using 14088-1-AP

IHC staining of human kidney using 14088-1-AP

IHC staining of human skeletal muscle using 14088-1-AP

IHC staining of human skeletal muscle using 14088-1-AP

IF Staining of HeLa using 14088-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

A metastasis map of human cancer cell lines.

Divergent transcriptional regulation of astrocyte reactivity across disorders.

TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis.

CircACC1 Regulates Assembly and Activation of AMPK Complex under Metabolic Stress.

Senescence-associated 13-HODE production promotes age-related liver steatosis by directly inhibiting catalase activity

PMID: 29729186

Reviews

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