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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SREBF1 Monoclonal antibody (66875-1-Ig)

    WB analysis using 66875-1-Ig (same clone as 66875-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66875-1-Ig (SREBF1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66875-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using SREBF1 Monoclonal antibody (66875-1-Ig)

    WB analysis of HeLa using 66875-1-Ig (same clone as 66875-1-PBS)

    WB result of SREBF1 antibody (66875-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SREBF1 transfected HeLa cells. This data was developed using the same antibody clone with 66875-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SREBF1 Monoclonal antibody (66875-1-Ig)

    WB analysis using 66875-1-Ig (same clone as 66875-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66875-1-Ig (SREBF1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66875-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SREBF1 Monoclonal antibody (66875-1-Ig)

    WB analysis using 66875-1-Ig (same clone as 66875-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66875-1-Ig (SREBF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66875-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using SREBF1 Monoclonal antibody (66875-1-Ig)

    IHC staining of human kidney using 66875-1-Ig (same clone as 66875-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66875-1-Ig (SREBF1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66875-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using SREBF1 Monoclonal antibody (66875-1-Ig)

    IHC staining of human kidney using 66875-1-Ig (same clone as 66875-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66875-1-Ig (SREBF1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66875-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SREBF1 Monoclonal antibody (66875-1-Ig)

    IF Staining of HepG2 using 66875-1-Ig (same clone as 66875-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SREBF1 antibody (66875-1-Ig, Clone: 1B6G5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). CL594-Phalloidin stains the F-actin (red). DAPI (blue). This data was developed using the same antibody clone with 66875-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SREBF1 Monoclonal antibody (66875-1-Ig)

    IF Staining of HepG2 using 66875-1-Ig (same clone as 66875-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SREBF1 antibody (66875-1-Ig, Clone: 1B6G5 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 66875-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SREBF1 Monoclonal antibody (66875-1-Ig)

    IF Staining of HepG2 using 66875-1-Ig (same clone as 66875-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66875-1-Ig (SREBF1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66875-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

SREBF1 Monoclonal antibody, PBS Only

SREBF1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66875-1-PBS
Clone No.1B6G5

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

SREBP 1, SREBF 1, Processed sterol regulatory element-binding protein 1, Class D basic helix-loop-helix protein 1, bHLHd1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66875-1-PBS targets SREBF1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SREBF1 fusion protein Ag5484 相同性解析による交差性が予測される生物種
Full Name sterol regulatory element binding transcription factor 1
Calculated molecular weight 1177 aa, 125 kDa
Observed molecular weight 125 kDa
GenBank accession numberBC063281
Gene Symbol SREBF1
Gene ID (NCBI) 6720
RRIDAB_2882209
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP36956
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SREBF1, also named as BHLHD1 and SREBP1, contains one basic helix-loop-helix (bHLH) domain and belongs to the SREBP family. It is a transcriptional activator required for lipid homeostasis. The SREBPs are synthesized as precursors anchored to endoplasmic reticulum (ER) membranes and complexed with SCAP. When the cellular cholesterol level is low, SREBP-SCAP complexes move to the Golgi apparatus, where SREBPs undergo a two-step proteolytic processing, leading to the release of the mature form, an N-terminal fragment, i.e, basic helix-loop-helix leucine zipper transcription factor. These factors enter the nucleus where they bind to sterol regulatory elements (SRE) in the promoter regions of a number of genes whose products mediate the synthesis of cholesterol and fatty acids. [PMID: 21698267]. This antibody can recognize the 125kd precursor form and the 68kd mature form of human SREBF1.