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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SREBF2 Polyclonal antibody (28212-1-AP)

    WB analysis using 28212-1-AP (same clone as 28212-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28212-1-AP (SREBF2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28212-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SREBF2 Polyclonal antibody (28212-1-AP)

    WB analysis using 28212-1-AP (same clone as 28212-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28212-1-AP (SREBF2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28212-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

SREBF2 Polyclonal antibody, PBS Only

SREBF2 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 28212-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

28212-1-PBS targets SREBF2 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SREBF2 fusion protein Ag28205 相同性解析による交差性が予測される生物種
Full Name sterol regulatory element binding transcription factor 2
Calculated molecular weight 124 kDa
Observed molecular weight73-75 kDa, 120-130 kDa
GenBank accession numberBC056158
Gene Symbol SREBF2
Gene ID (NCBI) 6721
RRIDAB_2881091
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ12772
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

SREBF2,also named as BHLHD2, is a 1141 amino acid protein, which contains 1 bHLH domain and belongs to the SREBP family. SREBF2 localizes in the endoplasmic reticulum membrane and is ubiquitously expressed in adult and fetal tissues. SREBF2 as a transcriptional activator is required for lipid homeostasis. SREBF2 expression is dynamically regulated in response to nutritional and hormonal cues. SREBF2 exists two isoform with molecular weight 124 kDa and 73 kDa.