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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SREBF2 Recombinant antibody

SREBF2 Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240451A12

Cat no : 83403-1-RR

Synonyms

SREBP 2, SREBF 2, Processed sterol regulatory element-binding protein 2, Class D basic helix-loop-helix protein 2, bHLHd2

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SREBF2 Recombinant antibody (83403-1-RR)

    IF Staining of HepG2 using 83403-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SREBF2 antibody (83403-1-RR, Clone: 240451A12 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SREBF2 Recombinant antibody (83403-1-RR)

    IF Staining of HepG2 using 83403-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SREBF2 antibody (83403-1-RR, Clone: 240451A12 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using SREBF2 Recombinant antibody (83403-1-RR)

    FC experiment of HeLa using 83403-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug SREBF2 Recombinant antibody (83403-1-RR, Clone:240451A12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83403-1-RR

    Affinity and Kinetic Characterization of 83403-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83403-1-RR against Human SREBF2 were performed. The affinity constant is 0.536 nM.

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Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83403-1-RR is SREBF2 Fusion Protein expressed in E. coli.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SREBF2 fusion protein Ag28205 相同性解析による交差性が予測される生物種
Full Name sterol regulatory element binding transcription factor 2
Calculated molecular weight 124 kDa
GenBank accession numberBC056158
Gene symbol SREBF2
Gene ID (NCBI) 6721
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SREBF2,also named as BHLHD2, is a 1141 amino acid protein, which contains 1 bHLH domain and belongs to the SREBP family. SREBF2 localizes in the endoplasmic reticulum membrane and is ubiquitously expressed in adult and fetal tissues. SREBF2 as a transcriptional activator is required for lipid homeostasis.

Protocols

Product Specific Protocols
IF protocol for SREBF2 antibody 83403-1-RRDownload protocol
FC protocol for SREBF2 antibody 83403-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols