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KD/KO Validated

SRF Polyclonal antibody

Name: SRF Polyclonal antibody
Brand: Proteintech
SKU: 16821-1-AP

SRF Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 16821-1-AP

Synonyms

Serum response factor, MCM1

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis of MCF-7 using 16821-1-AP
WB result of SRF antibody (16821-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SRF transfected MCF-7 cells.
WB analysis using 16821-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16821-1-AP (SRF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis using 16821-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16821-1-AP (SRF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 16821-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 16821-1-AP (SRF antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 16821-1-AP
IP result of anti-SRF (IP:16821-1-AP, 3ug; Detection:16821-1-AP 1:1000) with HeLa cells lysate 3000ug.
IHC staining of mouse heart using 16821-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16821-1-AP (SRF antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse heart using 16821-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16821-1-AP (SRF antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of A431 using 16821-1-AP
Immunofluorescent analysis of (4% PFA) fixed A431 cells using SRF antibody (16821-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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Cat No. 16821-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	A431 cells, MCF-7 cells, K-562 cells, HEK-293 cells, HeLa cells, NIH/3T3 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	mouse heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A431 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:2000-1:8000
Immunofluorescence (IF)/ICC	IF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 16 publications below
IHC	See 2 publications below
IF	See 2 publications below
IP	See 1 publications below
CoIP	See 2 publications below
ChIP	See 2 publications below

Product Information

16821-1-AP targets SRF in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SRF fusion protein Ag10386 相同性解析による交差性が予測される生物種
Full Name	serum response factor (c-fos serum response element-binding transcription factor)
Calculated molecular weight	508 aa, 52 kDa
Observed molecular weight	65-70 kDa, 40 kDa
GenBank accession number	BC048211
Gene symbol	SRF
Gene ID (NCBI)	6722
RRID	AB_2194384
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Serum response factor (SRF) is a transcription factor that binds the serum response element (SRE), a sequence that mediates the transient response of many cellular genes to growth stimulation. SRF is required for cardiac differentiation and maturation due to the role in cardiac cell growth and muscle gene regulation. The full-length SRF protein is 67 kDa and molecular weight of cleaved fragment is 50 kDa and 20 kDa (PMID: 21769134).

Protocols

Product Specific Protocols
WB protocol for SRF antibody 16821-1-AP	Download protocol
IHC protocol for SRF antibody 16821-1-AP	Download protocol
IF protocol for SRF antibody 16821-1-AP	Download protocol
IP protocol for SRF antibody 16821-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Exp Mol Med Helicobacter pylori CagA-mediated ether lipid biosynthesis promotes ferroptosis susceptibility in gastric cancer Authors - Yanmei Peng KD Validated View Article
human	IP	Am J Respir Cell Mol Biol SRF Expression in Excess Permits a Dual Contractile-Proliferative Phenotype of Airway Smooth Muscle Authors - Rui Sun View Article
	WB	Cell Physiol Biochem Galectin-3- Mediated Transdifferentiation of Pulmonary Artery Endothelial Cells Contributes to Hypoxic Pulmonary Vascular Remodeling. Authors - Li Zhang View Article
mouse	WB	Stem Cell Res Ther Activin B-activated Cdc42 signaling plays a key role in regulating adipose-derived mesenchymal stem cells-mediated skin wound healing. Authors - Simin Huang View Article
human	WB	Am J Physiol Lung Cell Mol Physiol Myocardin regulates fibronectin expression and secretion from human pleural mesothelial cells Authors - Tsuyoshi Sakai View Article
mouse	ChIP	J Immunol TPL-2 Inhibits IFN-β Expression via an ERK1/2-TCF-FOS Axis in TLR4-Stimulated Macrophages. Authors - Louise Blair View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SRF Polyclonal antibody

SRF Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of MCF-7 using 16821-1-AP

WB analysis using 16821-1-AP

WB analysis using 16821-1-AP

WB analysis of K-562 using 16821-1-AP

IP experiment of HeLa using 16821-1-AP

IHC staining of mouse heart using 16821-1-AP

IHC staining of mouse heart using 16821-1-AP

IF Staining of A431 using 16821-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Helicobacter pylori CagA-mediated ether lipid biosynthesis promotes ferroptosis susceptibility in gastric cancer

SRF Expression in Excess Permits a Dual Contractile-Proliferative Phenotype of Airway Smooth Muscle

Galectin-3- Mediated Transdifferentiation of Pulmonary Artery Endothelial Cells Contributes to Hypoxic Pulmonary Vascular Remodeling.

Activin B-activated Cdc42 signaling plays a key role in regulating adipose-derived mesenchymal stem cells-mediated skin wound healing.

Myocardin regulates fibronectin expression and secretion from human pleural mesothelial cells

TPL-2 Inhibits IFN-β Expression via an ERK1/2-TCF-FOS Axis in TLR4-Stimulated Macrophages.