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- KD/KO Validated
SRF Monoclonal antibody
SRF Monoclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, rat
Applications
WB, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
1G8F4
Cat no : 66742-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, LNCaP cells, MCF-7 cells, RAW 264.7 cells, HEK-293 cells, HepG2 cells, Jurkat cells, SK-BR-3 cells, IMR-32 cells, HSC-T6 cells, NIH/3T3 cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
Product Information
66742-1-Ig targets SRF in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | SRF fusion protein Ag10386 相同性解析による交差性が予測される生物種 |
| Full Name | serum response factor (c-fos serum response element-binding transcription factor) |
| Calculated molecular weight | 508 aa, 52 kDa |
| GenBank accession number | BC048211 |
| Gene symbol | SRF |
| Gene ID (NCBI) | 6722 |
| RRID | AB_2882090 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Serum response factor (SRF) is a transcription factor that binds the serum response element (SRE), a sequence that mediates the transient response of many cellular genes to growth stimulation. SRF is required for cardiac differentiation and maturation due to the role in cardiac cell growth and muscle gene regulation. The full-length SRF protein is 67 kDa and molecular weight of cleaved fragment is 50 kDa and 20 kDa (PMID: 21769134).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SRF antibody 66742-1-Ig | Download protocol |
| IF protocol for SRF antibody 66742-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Ther Nucleic Acids Diverging targets mediate the pathological roleof miR-199a-5p and miR-199a-3p by promoting cardiac hypertrophy and fibrosis | ||
bioRxiv Single molecule imaging of the central dogma reveals myosin-2A gene expression is regulated by contextual translational buffering
| ||
Mol Neurobiol SRF Facilitates Transcriptional Inhibition of Gem Expression by m6A Methyltransferase METTL3 to Suppress Neuronal Damage in Epilepsy |
