SRGAP1 Polyclonal antibody

Name: SRGAP1 Polyclonal antibody
Brand: Proteintech
SKU: 13252-1-AP

SRGAP1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 13252-1-AP

Synonyms

ARHGAP13, KIAA1304, SRGAP1

Validation Data Gallery

Filter:

AllWBIPIHC

WB analysis using 13252-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 13252-1-AP (SRGAP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 13252-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13252-1-AP (SRGAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 13252-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 13252-1-AP (SRGAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of SH-SY5Y using 13252-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 13252-1-AP (SRGAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Apoptosised HeLa using 13252-1-AP
Apoptosised HeLa cells were subjected to SDS PAGE followed by western blot with 13252-1-AP (SRGAP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 13252-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 13252-1-AP (SRGAP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IP experiment of mouse kidney using 13252-1-AP
IP result of anti-SRGAP1 (IP:13252-1-AP, 4ug; Detection:13252-1-AP 1:500) with mouse kidney tissue lysate 4000ug.
IHC staining of human liver cancer using 13252-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13252-1-AP (SRGAP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 13252-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13252-1-AP (SRGAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 13252-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13252-1-AP (SRGAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 13252-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13252-1-AP (SRGAP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 13252-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13252-1-AP (SRGAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 13252-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13252-1-AP (SRGAP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 13252-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, mouse brain tissue, Apoptosised HeLa cells, mouse kidney tissue, HEK-293 cells, SH-SY5Y cells
Positive IP detected in	mouse kidney tissue
Positive IHC detected in	human liver cancer tissue, mouse testis tissue, human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below
IF	See 2 publications below
IP	See 1 publications below

Product Information

13252-1-AP targets SRGAP1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SRGAP1 fusion protein Ag4104 相同性解析による交差性が予測される生物種
Full Name	SLIT-ROBO Rho GTPase activating protein 1
Calculated molecular weight	1085 aa, 124 kDa
Observed molecular weight	124 kDa
GenBank accession number	BC029919
Gene symbol	SRGAP1
Gene ID (NCBI)	57522
RRID	AB_2270916
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for SRGAP1 antibody 13252-1-AP	Download protocol
IHC protocol for SRGAP1 antibody 13252-1-AP	Download protocol
IP protocol for SRGAP1 antibody 13252-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB,IF,IP	J Am Soc Nephrol SRGAP1 Controls Small Rho GTPases To Regulate Podocyte Foot Process Maintenance. Authors - Manuel Rogg View Article
human	WB	J Exp Clin Cancer Res srGAP1 mediates the migration inhibition effect of Slit2-Robo1 in colorectal cancer. Authors - Yuyang Feng View Article
human	WB,IF	Cell Rep A proliferative to invasive switch is mediated by srGAP1 downregulation through the activation of TGF-β2 signaling Authors - Chandrani Mondal View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SRGAP1 Polyclonal antibody

SRGAP1 Polyclonal Antibody for WB, IP, IHC, ELISA

Validation Data Gallery

WB analysis using 13252-1-AP

WB analysis of mouse kidney using 13252-1-AP

WB analysis of HEK-293 using 13252-1-AP

WB analysis of SH-SY5Y using 13252-1-AP

WB analysis of Apoptosised HeLa using 13252-1-AP

WB analysis of mouse brain using 13252-1-AP

IP experiment of mouse kidney using 13252-1-AP

IHC staining of human liver cancer using 13252-1-AP

IHC staining of human liver cancer using 13252-1-AP

IHC staining of human kidney using 13252-1-AP

IHC staining of mouse testis using 13252-1-AP