- Featured Product
- KD/KO Validated
SRGAP2 Polyclonal antibody
SRGAP2 Polyclonal Antibody for WB, IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IP, ELISA, IF
Conjugate
Unconjugated
Cat no : 22519-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse kidney tissue, HEK-293 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | mouse brain tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
| IHC | See 2 publications below |
| IF | See 1 publications below |
Product Information
22519-1-AP targets SRGAP2 in WB, IHC, IP, ELISA, IF applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SRGAP2 fusion protein Ag18278 相同性解析による交差性が予測される生物種 |
| Full Name | SLIT-ROBO Rho GTPase activating protein 2 |
| Calculated molecular weight | 1071 aa, 121 kDa |
| Observed molecular weight | 121 kDa |
| GenBank accession number | BC132874 |
| Gene symbol | SRGAP2 |
| Gene ID (NCBI) | 23380 |
| RRID | AB_2879114 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SLIT-ROBO Rho GTPase-activating protein 2 (SRGAP2) is also named as ARHGAP34, FNBP2, KIAA0456 and SRGAP2A. SRGAP2 plays important roles in axon guidance, neuronal migration, synapse formation, and nerve regeneration. SRGAP2 protein was first expressed in the retina of normal mice at the embryonic stage and was mainly located in the mature retinal ganglion cell layer and the inner nuclear layer (PMID: 37056152). SRGAP2 plays a key role in neuronal morphogenesis and migration mainly during development of the cerebral cortex (PMID: 20810653, PMID: 27373832, PMID: 28333212). SRGAP2 are critical for neuronal migration through inactivation of Rho GTPases Cdc42, Rac1, and RhoA (PMID: 20810653). SRGAP2A promotes synaptic maturation, and ultimately the synaptic accumulation of AMPA and GABAA receptors, by interacting with key components of both excitatory and inhibitory postsynaptic scaffolds, Homer and Gephyrin (PMID: 27373832).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SRGAP2 antibody 22519-1-AP | Download protocol |
| IHC protocol for SRGAP2 antibody 22519-1-AP | Download protocol |
| IP protocol for SRGAP2 antibody 22519-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Theranostics Stiff matrix induced srGAP2 tension gradients control migration direction in triple-negative breast cancer
| ||
Nat Commun Haploinsufficiency in the ANKS1B gene encoding AIDA-1 leads to a neurodevelopmental syndrome. | ||
Cell Rep Huntingtin coordinates dendritic spine morphology and function through cofilin-mediated control of the actin cytoskeleton | ||
Biomed Pharmacother CDDO-Me ameliorates podocyte injury through anti-oxidative stress and regulation of actin cytoskeleton in adriamycin nephropathy |
