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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SRGAP3 Polyclonal antibody

SRGAP3 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 29624-1-AP

Synonyms

SRGAP 3, SLIT-ROBO Rho GTPase-activating protein 3, SLIT ROBO Rho GTPase activating protein 3, Rho GTPase-activating protein 14, Mental disorder-associated GAP

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SRGAP3 Polyclonal antibody (29624-1-AP)

    WB analysis using 29624-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29624-1-AP (SRGAP3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using SRGAP3 Polyclonal antibody (29624-1-AP)

    IHC staining of human ovary cancer using 29624-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 29624-1-AP (SRGAP3 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using SRGAP3 Polyclonal antibody (29624-1-AP)

    IF Staining of mouse brain using 29624-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SRGAP3 antibody (29624-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29624-1-AP targets SRGAP3 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SRGAP3 fusion protein Ag31196 相同性解析による交差性が予測される生物種
Full Name SLIT-ROBO Rho GTPase activating protein 3
Calculated molecular weight 124 kDa
Observed molecular weight125 kDa
GenBank accession numberNM_014850
Gene symbol SRGAP3
Gene ID (NCBI) 9901
RRIDAB_2918334
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SRGAP3 (SLIT-ROBO Rho GTPase Activating Protein 3), also named MEGAP and WRP, is a member of the Slit-Robo sub-family of Rho GTPase-activating proteins (Rho GAPs), controls actin and microtubule dynamics through negative regulation of Rac(PMID: 23108406). Diseases associated with SRGAP3 include Pilomyxoid Astrocytoma and Avoidant Personality Disorder. SRGAP3 is highly expressed in fetal and adult brain, including the cortex and the hippocampus.

Protocols

Product Specific Protocols
WB protocol for SRGAP3 antibody 29624-1-APDownload protocol
IHC protocol for SRGAP3 antibody 29624-1-APDownload protocol
IF protocol for SRGAP3 antibody 29624-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols