Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of mouse liver using 16471-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 16471-1-AP (SRI Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of rat liver using 16471-1-AP
rat liver tissue were subjected to SDS PAGE followed by western blot with 16471-1-AP (SRI Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 16471-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 16471-1-AP (SRI antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 16471-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 16471-1-AP (SRI antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HepG2 using 16471-1-AP
IP result of anti-SRI (IP:16471-1-AP, 3ug; Detection:16471-1-AP 1:500) with HepG2 cells lysate 600ug.
IHC staining of human liver using 16471-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16471-1-AP (SRI antibody) at dilution of 1:500 (under 10x lens).
IHC staining of human liver using 16471-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16471-1-AP (SRI antibody) at dilution of 1:500 (under 40x lens).
IF Staining of HepG2 using 16471-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SRI antibody (16471-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

SRI Polyclonal antibody

SRI Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 16471-1-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

22 kDa protein, CP 22, SCN, sorcin, SRI, V19

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (6)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse liver tissue, HepG2 cells, human liver tissue, rat liver tissue
Positive IP detected in	HepG2 cells
Positive IHC detected in	human liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 6 publications below
IHC	See 2 publications below
IF	See 3 publications below
IP	See 1 publications below
CoIP	See 1 publications below

Product Information

16471-1-AP targets SRI in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SRI fusion protein Ag9553 相同性解析による交差性が予測される生物種
Full Name	sorcin
Calculated molecular weight	198 aa, 22 kDa
Observed molecular weight	22 kDa
GenBank accession number	BC011025
Gene Symbol	SRI
Gene ID (NCBI)	6717
RRID	AB_2255255
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P30626
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for SRI antibody 16471-1-AP	Download protocol
IHC protocol for SRI antibody 16471-1-AP	Download protocol
IF protocol for SRI antibody 16471-1-AP	Download protocol
IP protocol for SRI antibody 16471-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	J Cell Physiol Targeting P-glycoprotein and SORCIN: Dihydromyricetin strengthens anti-proliferative efficiency of adriamycin via MAPK/ERK and Ca(2+) -mediated apoptosis pathways in MCF-7/ADR and K562/ADR. Authors - Yaoting Sun View Article
human	WB	J Cell Physiol PMID: 30171603 Authors - Yaoting Sun View Article
mouse	IF, WB	Arch Biochem Biophys Knockdown of sorcin increases HEI-OC1 cell damage induced by cisplatin in vitro. Authors - Dan Wang View Article
human	WB,IHC,IF,IP	Cell Death Dis AnnexinA7 promotes epithelial-mesenchymal transition by interacting with Sorcin and contributes to aggressiveness in hepatocellular carcinoma. Authors - Fei Ling View Article
human	WB	J Physiol Biochem Sorcin promotes proliferation of hepatocellular carcinoma by regulating VEGFA/B via PI3K pathway Authors - Huan Zhang View Article
human,mouse	WB,IHC,IF,CoIP	Int J Mol Sci Sorcin Inhibits Mitochondrial Apoptosis by Interacting with STAT3 via NF-κB Pathway Authors - Yizi Li View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of mouse liver using 16471-1-AP

WB analysis of rat liver using 16471-1-AP

WB analysis of HepG2 using 16471-1-AP

WB analysis of human liver using 16471-1-AP

IP experiment of HepG2 using 16471-1-AP

IHC staining of human liver using 16471-1-AP

IHC staining of human liver using 16471-1-AP

IF Staining of HepG2 using 16471-1-AP

SRI Polyclonal antibody

SRI Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Targeting P-glycoprotein and SORCIN: Dihydromyricetin strengthens anti-proliferative efficiency of adriamycin via MAPK/ERK and Ca(2+) -mediated apoptosis pathways in MCF-7/ADR and K562/ADR.

PMID: 30171603

Knockdown of sorcin increases HEI-OC1 cell damage induced by cisplatin in vitro.

AnnexinA7 promotes epithelial-mesenchymal transition by interacting with Sorcin and contributes to aggressiveness in hepatocellular carcinoma.

Sorcin promotes proliferation of hepatocellular carcinoma by regulating VEGFA/B via PI3K pathway

Sorcin Inhibits Mitochondrial Apoptosis by Interacting with STAT3 via NF-κB Pathway