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- KD/KO Validated
SRP14 Polyclonal antibody
SRP14 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat and More (1)
Applications
WB, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 11528-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, HeLa cells, human heart tissue |
| Positive IP detected in | A549 cells |
| Positive IF/ICC detected in | MCF-7 cells, HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 12 publications below |
| IF | See 1 publications below |
| IP | See 2 publications below |
Product Information
11528-1-AP targets SRP14 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SRP14 fusion protein Ag2099 相同性解析による交差性が予測される生物種 |
| Full Name | signal recognition particle 14kDa (homologous Alu RNA binding protein) |
| Calculated molecular weight | 136 aa, 15 kDa |
| Observed molecular weight | 18 kDa |
| GenBank accession number | BC035495 |
| Gene symbol | SRP14 |
| Gene ID (NCBI) | 6727 |
| RRID | AB_2194708 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Signal recognition particle(SRP) acts in three distinct ways: 1 it binds to the signal sequence of the nascent polypeptide to be translocated, which is exposed on the surface of the translating ribosome; 2 it temporarily retards the nascent polypeptide from further elongation; 3 it mediates docking of the SRP-ribosome-nascent polypeptide chain complex to the RER membrane via the heterodimeric SRP-receptor (SR). SRP14, also named as 18 kDa Alu RNA-binding protein, is a 136 amino acid protein. Signal recognition particle consists of a 7S RNA molecule of 300 nucleotides and six protein subunits: SRP72, SRP68, SRP54, SRP19, SRP14 and SRP9. SRP14 protein is a component of signal-recognition-particle that has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum membrane. SRP9 together with SRP14 and the Alu portion of the SRP RNA, constitutes the elongation arrest domain of SRP. SRP14 form a heterodimer with SRP9, which recognizes Alu RNA and the related 7SL RNA.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SRP14 antibody 11528-1-AP | Download protocol |
| IF protocol for SRP14 antibody 11528-1-AP | Download protocol |
| IP protocol for SRP14 antibody 11528-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Exosome RNA Unshielding Couples Stromal Activation to Pattern Recognition Receptor Signaling in Cancer.
| ||
Mol Cell Estrogen-Related Hormones Induce Apoptosis by Stabilizing Schlafen-12 Protein Turnover. | ||
Genes Dev Human intron-encoded Alu RNAs are processed and packaged into Wdr79-associated nucleoplasmic box H/ACA RNPs. | ||
Nucleic Acids Res Comprehensive analysis of the BC200 ribonucleoprotein reveals a reciprocal regulatory function with CSDE1/UNR. | ||
Nucleic Acids Res Implication of the SMN complex in the biogenesis and steady state level of the signal recognition particle. | ||
