SRP54 Polyclonal antibody

Name: SRP54 Polyclonal antibody
Brand: Proteintech
SKU: 11729-1-AP

SRP54 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 11729-1-AP

Synonyms

Signal recognition particle subunit SRP54, SCN8, EC:3.6.5.4

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis of human placenta using 11729-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 11729-1-AP (SRP54 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 11729-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11729-1-AP (SRP54 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 11729-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 11729-1-AP (SRP54 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IP experiment of human placenta using 11729-1-AP
IP result of anti-SRP54 (IP:11729-1-AP, 4ug; Detection:11729-1-AP 1:300) with human placenta tissue lysate 1015 ug.
IHC staining of human breast cancer using 11729-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11729-1-AP (SRP54 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 11729-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11729-1-AP (SRP54 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 11729-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11729-1-AP (SRP54 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 11729-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11729-1-AP (SRP54 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 11729-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11729-1-AP (SRP54 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 11729-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11729-1-AP (SRP54 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 11729-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11729-1-AP (SRP54 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 11729-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11729-1-AP (SRP54 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 11729-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SRP54 antibody (11729-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Cat No. 11729-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	human placenta tissue, NIH/3T3 cells
Positive IP detected in	human placenta tissue
Positive IHC detected in	human breast cancer tissue, human placenta tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 4 publications below

Product Information

11729-1-AP targets SRP54 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SRP54 fusion protein Ag2327 相同性解析による交差性が予測される生物種
Full Name	signal recognition particle 54kDa
Calculated molecular weight	54 kDa
Observed molecular weight	54 kDa
GenBank accession number	BC003389
Gene symbol	SRP54
Gene ID (NCBI)	6729
RRID	AB_2194724
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The signal recognition particle (SRP) is a ribonucleoprotein complex that mediates the targeting of proteins to the endoplasmic reticulum (ER). The complex consists of a 7S (or 7SL) RNA and 6 different proteins, and signal recognition particle 54 (SRP54) is one of them. SRP54 binds to the signal sequence of presecretory protein as they emerge from the translating ribosomes, and then transfers them to translocating chain-associating membrane protein (TRAM).

Protocols

Product Specific Protocols
WB protocol for SRP54 antibody 11729-1-AP	Download protocol
IHC protocol for SRP54 antibody 11729-1-AP	Download protocol
IF protocol for SRP54 antibody 11729-1-AP	Download protocol
IP protocol for SRP54 antibody 11729-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human		Mol Cell Estrogen-Related Hormones Induce Apoptosis by Stabilizing Schlafen-12 Protein Turnover. Authors - Dianrong Li View Article
human	WB	PLoS One Decreased dicer expression enhances SRP-mediated protein targeting. Authors - Ren Yong-Feng YF View Article
human	WB	Biochem Biophys Res Commun Polyglutamine expansion disturbs the endoplasmic reticulum formation, leading to caspase-7 activation through Bax. Authors - Masashi Ueda View Article
human	WB	Biochem Biophys Res Commun Expanded polyglutamine embedded in the endoplasmic reticulum causes membrane distortion and coincides with Bax insertion. Authors - Masashi Ueda View Article
human	WB	iScience Differential toxicity and localization of arginine-rich C9ORF72 dipeptide repeat proteins depend on de-clustering of positive charges Authors - Tamami Miyagi View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SRP54 Polyclonal antibody

SRP54 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of human placenta using 11729-1-AP

WB analysis of NIH/3T3 using 11729-1-AP

WB analysis of human placenta using 11729-1-AP

IP experiment of human placenta using 11729-1-AP

IHC staining of human breast cancer using 11729-1-AP

IHC staining of human placenta using 11729-1-AP

IHC staining of human placenta using 11729-1-AP

IHC staining of human breast cancer using 11729-1-AP

IHC staining of human breast cancer using 11729-1-AP

IHC staining of human placenta using 11729-1-AP

IHC staining of human placenta using 11729-1-AP

IHC staining of human breast cancer using 11729-1-AP

IF Staining of HepG2 using 11729-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Estrogen-Related Hormones Induce Apoptosis by Stabilizing Schlafen-12 Protein Turnover.

Decreased dicer expression enhances SRP-mediated protein targeting.

Polyglutamine expansion disturbs the endoplasmic reticulum formation, leading to caspase-7 activation through Bax.

Expanded polyglutamine embedded in the endoplasmic reticulum causes membrane distortion and coincides with Bax insertion.

Differential toxicity and localization of arginine-rich C9ORF72 dipeptide repeat proteins depend on de-clustering of positive charges