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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SRP54 Polyclonal antibody

SRP54 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 17976-1-AP

Synonyms

Signal recognition particle subunit SRP54, SCN8, EC:3.6.5.4

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using SRP54 Polyclonal antibody (17976-1-AP)

    WB analysis of HeLa using 17976-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17976-1-AP (SRP54 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using SRP54 Polyclonal antibody (17976-1-AP)

    WB analysis of NIH/3T3 using 17976-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 17976-1-AP (SRP54 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using SRP54 Polyclonal antibody (17976-1-AP)

    IP experiment of HeLa using 17976-1-AP

    IP result of anti-SRP54 (IP:17976-1-AP, 4ug; Detection:17976-1-AP 1:500) with HeLa cells lysate 1600ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SRP54 Polyclonal antibody (17976-1-AP)

    IHC staining of human breast cancer using 17976-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17976-1-AP (SRP54 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SRP54 Polyclonal antibody (17976-1-AP)

    IHC staining of human breast cancer using 17976-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17976-1-AP (SRP54 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SRP54 Polyclonal antibody (17976-1-AP)

    IF Staining of HepG2 using 17976-1-AP

    Immunofluorescent analysis of HepG2 cells, using SRP54 antibody 17976-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

Positive WB detected inHeLa cells, NIH/3T3 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17976-1-AP targets SRP54 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SRP54 fusion protein Ag12166 相同性解析による交差性が予測される生物種
Full Name signal recognition particle 54kDa
Calculated molecular weight 54 kDa
Observed molecular weight 54 kDa
GenBank accession numberBC003389
Gene symbol SRP54
Gene ID (NCBI) 6729
RRIDAB_2194722
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The signal recognition particle (SRP) is a ribonucleoprotein complex that mediates the targeting of proteins to the endoplasmic reticulum (ER). The complex consists of a 7S (or 7SL) RNA and 6 different proteins, and signal recognition particle 54 (SRP54) is one of them. SRP54 binds to the signal sequence of presecretory protein as they emerge from the translating ribosomes, and then transfers them to translocating chain-associating membrane protein (TRAM). This antibody specifically recognizes the 54kd SRP54 protein.

Protocols

Product Specific Protocols
WB protocol for SRP54 antibody 17976-1-APDownload protocol
IHC protocol for SRP54 antibody 17976-1-APDownload protocol
IF protocol for SRP54 antibody 17976-1-APDownload protocol
IP protocol for SRP54 antibody 17976-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP

Nucleic Acids Res

Implication of the SMN complex in the biogenesis and steady state level of the signal recognition particle.

Authors - Piazzon Nathalie N
View Article