SRP54 Monoclonal antibody
SRP54 Monoclonal Antibody for WB, IHC, ELISA, FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat
Applications
WB, IHC, ELISA, FC (Intra)
Conjugate
Unconjugated
CloneNo.
1D6D1
Cat no : 67005-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, HEK-293 cells, MCF-7 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
67005-1-Ig targets SRP54 in WB, IHC, ELISA, FC (Intra) applications and shows reactivity with Human, mouse, rat samples.
Tested Reactivity | Human, mouse, rat |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | SRP54 fusion protein Ag12166 相同性解析による交差性が予測される生物種 |
Full Name | signal recognition particle 54kDa |
Calculated molecular weight | 54 kDa |
Observed molecular weight | 54 kDa |
GenBank accession number | BC003389 |
Gene symbol | SRP54 |
Gene ID (NCBI) | 6729 |
RRID | AB_2882322 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The signal recognition particle (SRP) is a ribonucleoprotein complex that mediates the targeting of proteins to the endoplasmic reticulum (ER). The complex consists of a 7S (or 7SL) RNA and 6 different proteins, and signal recognition particle 54 (SRP54) is one of them. SRP54 binds to the signal sequence of presecretory protein as they emerge from the translating ribosomes, and then transfers them to translocating chain-associating membrane protein (TRAM).
Protocols
Product Specific Protocols | |
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WB protocol for SRP54 antibody 67005-1-Ig | Download protocol |
IHC protocol for SRP54 antibody 67005-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |