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Target

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Species Reactivity

Species Reactivity

Conjugate

Conjugate

SRP54 Monoclonal antibody

SRP54 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1D6D1

Cat no : 67005-1-Ig

Synonyms

Signal recognition particle subunit SRP54, SCN8, EC:3.6.5.4, 1D6D1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SRP54 Monoclonal antibody (67005-1-Ig)

    WB analysis using 67005-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67005-1-Ig (SRP54 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SRP54 Monoclonal antibody (67005-1-Ig)

    IHC staining of human breast cancer using 67005-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67005-1-Ig (SRP54 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SRP54 Monoclonal antibody (67005-1-Ig)

    IHC staining of human breast cancer using 67005-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67005-1-Ig (SRP54 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SRP54 Monoclonal antibody (67005-1-Ig)

    IF Staining of HepG2 using 67005-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SRP54 antibody (67005-1-Ig, Clone: 1D6D1 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using SRP54 Monoclonal antibody (67005-1-Ig)

    FC experiment of HeLa using 67005-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human SRP54 (67005-1-Ig, Clone:1D6D1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, MCF-7 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67005-1-Ig targets SRP54 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SRP54 fusion protein Ag12166 相同性解析による交差性が予測される生物種
Full Name signal recognition particle 54kDa
Calculated molecular weight 54 kDa
Observed molecular weight 54 kDa
GenBank accession numberBC003389
Gene symbol SRP54
Gene ID (NCBI) 6729
RRIDAB_2882322
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The signal recognition particle (SRP) is a ribonucleoprotein complex that mediates the targeting of proteins to the endoplasmic reticulum (ER). The complex consists of a 7S (or 7SL) RNA and 6 different proteins, and signal recognition particle 54 (SRP54) is one of them. SRP54 binds to the signal sequence of presecretory protein as they emerge from the translating ribosomes, and then transfers them to translocating chain-associating membrane protein (TRAM).

Protocols

Product Specific Protocols
WB protocol for SRP54 antibody 67005-1-IgDownload protocol
IHC protocol for SRP54 antibody 67005-1-IgDownload protocol
IF protocol for SRP54 antibody 67005-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols