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  • Featured Product
  • KD/KO Validated

SRPK2 Polyclonal antibody

SRPK2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25417-1-AP

Synonyms

SFRS protein kinase 2, SFRSK2, SR protein specific kinase 2, SRPK2

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using SRPK2 Polyclonal antibody (25417-1-AP)

    WB analysis of HeLa using 25417-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25417-1-AP (SRPK2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using SRPK2 Polyclonal antibody (25417-1-AP)

    WB analysis of HeLa using 25417-1-AP

    WB result of SRPK2 antibody (25417-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-SRPK2 transfected HeLa cells.

  • Western Blot (WB) analysis of HeLa cells using SRPK2 Polyclonal antibody (25417-1-AP)

    WB analysis of HeLa using 25417-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25417-1-AP (SRPK2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using SRPK2 Polyclonal antibody (25417-1-AP)

    IP experiment of HeLa using 25417-1-AP

    IP result of anti-SRPK2 (IP:25417-1-AP, 4ug; Detection:25417-1-AP 1:300) with HeLa cells lysate 3200ug.

  • Immunoprecipitation (IP) experiment of HeLa cells using SRPK2 Polyclonal antibody (25417-1-AP)

    IP experiment of HeLa using 25417-1-AP

    IP result of anti-SRPK2 (IP:25417-1-AP, 4ug; Detection:25417-1-AP 1:300) with HeLa cells lysate 3200ug.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using SRPK2 Polyclonal antibody (25417-1-AP)

    IHC staining of mouse testis using 25417-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25417-1-AP (SRPK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using SRPK2 Polyclonal antibody (25417-1-AP)

    IHC staining of mouse testis using 25417-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25417-1-AP (SRPK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SRPK2 Polyclonal antibody (25417-1-AP)

    IHC staining of mouse brain using 25417-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25417-1-AP (SRPK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SRPK2 Polyclonal antibody (25417-1-AP)

    IHC staining of mouse brain using 25417-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25417-1-AP (SRPK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SRPK2 Polyclonal antibody (25417-1-AP)

    IHC staining of mouse brain using 25417-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25417-1-AP (SRPK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse testis tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25417-1-AP targets SRPK2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SRPK2 fusion protein Ag17885 相同性解析による交差性が予測される生物種
Full Name SFRS protein kinase 2
Calculated molecular weight 688 aa, 78 kDa
Observed molecular weight 105-110 kDa
GenBank accession numberBC035214
Gene symbol SRPK2
Gene ID (NCBI) 6733
RRIDAB_2880068
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SRPK2, also named as serine/arginine protein kinase 2 and SFRSK2, belongs to SR protein-specific kinase family which phosphorylate the serine/arginine domain containing proteins in the nuclear speckles to mediate pre-mRNA splicing (PMID: 22854045). In addition to its major role as kinase for the phosphorylation of SR proteins, SRPK2 is involved in cell cycle regulation and cell apoptosis (PMID: 19592491). SRPK2 promotes the proliferation and migration of the colon cancer cells. It has been reported to be regulated in several cancer types (PMID: 27041240). It has 2 isoforms with the calculated molecular mass of 77-79 kDa, and the apparent molecular mass of 105-115 kDa due to posttranslational modification (PMID: 12134018, 16122776).

Protocols

Product Specific Protocols
WB protocol for SRPK2 antibody 25417-1-APDownload protocol
IHC protocol for SRPK2 antibody 25417-1-APDownload protocol
IP protocol for SRPK2 antibody 25417-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Cancer Discov

Stress Granules determine the Development of Obesity-associated Pancreatic Cancer.

Authors - Guillaume Fonteneau
View Article