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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SRPR Polyclonal antibody

SRPR Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12090-1-AP

Synonyms

Docking protein alpha, DP, DP alpha, SR alpha, Sralpha, SRP alpha, SRPR, SRPR alpha

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using SRPR Polyclonal antibody (12090-1-AP)

    WB analysis of mouse liver using 12090-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 12090-1-AP (SRPR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using SRPR Polyclonal antibody (12090-1-AP)

    WB analysis of MCF-7 using 12090-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 12090-1-AP (SRPR antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SRPR Polyclonal antibody (12090-1-AP)

    IHC staining of human breast cancer using 12090-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 12090-1-AP (SRPR antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SRPR Polyclonal antibody (12090-1-AP)

    IHC staining of human breast cancer using 12090-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 12090-1-AP (SRPR antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inmouse liver tissue, MCF-7 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

12090-1-AP targets SRPR in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SRPR fusion protein Ag2730 相同性解析による交差性が予測される生物種
Full Name signal recognition particle receptor (docking protein)
Calculated molecular weight 638 aa, 70 kDa
Observed molecular weight 65-70 kDa
GenBank accession numberBC013583
Gene symbol SRPR
Gene ID (NCBI) 6734
RRIDAB_10596631
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SRPR antibody 12090-1-APDownload protocol
IHC protocol for SRPR antibody 12090-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

EBioMedicine

PMID: 29735414

Authors - Song-Yang Zhang
View Article