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Validation Data Gallery

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  • Western Blot (WB) analysis of pig lung tissue using SRPX2 Monoclonal antibody (66266-1-Ig)

    WB analysis of pig lung using 66266-1-Ig (same clone as 66266-1-PBS)

    pig lung tissue were subjected to SDS PAGE followed by western blot with 66266-1-Ig (SRPX2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66266-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using SRPX2 Monoclonal antibody (66266-1-Ig)

    WB analysis of A549 using 66266-1-Ig (same clone as 66266-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 66266-1-Ig (SRPX2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66266-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SRPX2 Monoclonal antibody (66266-1-Ig)

    IHC staining of mouse brain using 66266-1-Ig (same clone as 66266-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66266-1-Ig (SRPX2 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66266-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SRPX2 Monoclonal antibody (66266-1-Ig)

    IHC staining of mouse brain using 66266-1-Ig (same clone as 66266-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66266-1-Ig (SRPX2 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66266-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using SRPX2 Monoclonal antibody (66266-1-Ig)

    IF Staining of A549 using 66266-1-Ig (same clone as 66266-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using SRPX2 antibody (66266-1-Ig, Clone: 2A1D7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66266-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

SRPX2 Monoclonal antibody, PBS Only

SRPX2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66266-1-PBS
Clone No.2A1D7

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

BPP, CBPS, PMGX, RESDX, SRPUL, SRPX2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66266-1-PBS targets SRPX2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, pig, mouse samples.

Tested Reactivity human, pig, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen SRPX2 fusion protein Ag8084 相同性解析による交差性が予測される生物種
Full Name sushi-repeat-containing protein, X-linked 2
Calculated molecular weight 465 aa, 53 kDa
Observed molecular weight 53 kDa
GenBank accession numberBC020733
Gene Symbol SRPX2
Gene ID (NCBI) 27286
RRIDAB_2721268
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO60687
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

SRPX2 (sushi-repeat-containing protein, X-linked 2) is a secreted protein expressed in neurons of the human adult brain, including the rolandic area (PMID: 20874700). Firstly described as sushi-repeat protein up-regulated in leukemia (SPRUL) in leukemia cells with dysregulated expression at the transcriptional level, SRPX2 has been recently shown as a multifunctional protein. SRPX2 is a ligand for uPAR, the urokinase-type plasminogen activator (uPA) receptor (PMID: 18718938). It is involved in seizure disorders, angiogenesis and cellular adhesion (PMID: 22242148; 19667118; 16497722). The involvement of SRPX2 in disorders of language cortex suggests it has an important role in the perisylvian region critical for language and cognitive development (PMID: 16497722).