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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SRR Polyclonal antibody

SRR Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 17955-1-AP

Synonyms

ILV1, ISO1, serine racemase, SRR

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse brain tissue using SRR Polyclonal antibody (17955-1-AP)

    WB analysis of mouse brain using 17955-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 17955-1-AP (SRR Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using SRR Polyclonal antibody (17955-1-AP)

    WB analysis of mouse brain using 17955-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 17955-1-AP (SRR Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using SRR Polyclonal antibody (17955-1-AP)

    WB analysis of rat brain using 17955-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 17955-1-AP (SRR Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using SRR Polyclonal antibody (17955-1-AP)

    WB analysis of mouse brain using 17955-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 17955-1-AP (SRR Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using SRR Polyclonal antibody (17955-1-AP)

    IP experiment of mouse brain using 17955-1-AP

    IP result of anti-SRR (IP:17955-1-AP, 4ug; Detection:17955-1-AP 1:600) with mouse brain tissue lysate 4000 ug.

  • Immunohistochemistry (IHC) staining of human kidney tissue using SRR Polyclonal antibody (17955-1-AP)

    IHC staining of human kidney using 17955-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17955-1-AP (SRR Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using SRR Polyclonal antibody (17955-1-AP)

    IHC staining of human kidney using 17955-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17955-1-AP (SRR Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using SRR Polyclonal antibody (17955-1-AP)

    IHC staining of human heart using 17955-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 17955-1-AP (SRR Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using SRR Polyclonal antibody (17955-1-AP)

    IHC staining of human heart using 17955-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 17955-1-AP (SRR Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman kidney tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

The immunogen of 17955-1-AP is SRR Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SRR fusion protein Ag12449 相同性解析による交差性が予測される生物種
Full Name serine racemase
Calculated molecular weight 340 aa, 37 kDa
Observed molecular weight 37 kDa
GenBank accession numberBC074728
Gene symbol SRR
Gene ID (NCBI) 63826
RRIDAB_2878471
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SRR(serine racemase) is a 37 kDa protein and belongs to the serine/threonine dehydratase family. It plays a role in neuronal activity and a potential biological role in the regulation of N-methyl-D-aspartate (NMDA) receptor activity in these peripheral organs as well. SRR catalyzes the racemization between l- and d-serine.

Protocols

Product Specific Protocols
WB protocol for SRR antibody 17955-1-APDownload protocol
IHC protocol for SRR antibody 17955-1-APDownload protocol
IP protocol for SRR antibody 17955-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Neurosci

Selective ablation of Sod2 in astrocytes induces sex-specific effects on cognitive function, D-serine availability, and astrogliosis.

Authors - Matthew P Baier
View Article
mouseWB

Prog Neuropsychopharmacol Biol Psychiatry

Probing cognitive flexibility in Shank2-deficient mice: Effects of D-cycloserine and NMDAR signaling hub dynamics

Authors - Samia Afzal
View Article