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  • Featured Product
  • KD/KO Validated

SRRM1 Polyclonal antibody

SRRM1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 12822-1-AP

Synonyms

160 KD, POP101, SRM160, SRRM1

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using SRRM1 Polyclonal antibody (12822-1-AP)

    WB analysis of mouse liver using 12822-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 12822-1-AP (SRRM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SRRM1 Polyclonal antibody (12822-1-AP)

    WB analysis using 12822-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12822-1-AP (SRRM1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using SRRM1 Polyclonal antibody (12822-1-AP)

    WB analysis of HeLa using 12822-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12822-1-AP (SRRM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of UV treated HEK-293 using SRRM1 Polyclonal antibody (12822-1-AP)

    WB analysis of UV treated HEK-293 using 12822-1-AP

    UV treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 12822-1-AP (SRRM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using SRRM1 Polyclonal antibody (12822-1-AP)

    WB analysis of Jurkat using 12822-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 12822-1-AP (SRRM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using SRRM1 Polyclonal antibody (12822-1-AP)

    IP experiment of HeLa using 12822-1-AP

    IP result of anti-SRRM1 (IP:12822-1-AP, 4ug; Detection:12822-1-AP 1:300) with HeLa cells lysate 3200ug.

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Tested Applications

Positive WB detected inmouse liver tissue, HeLa cells, Jurkat cells, UV treated HEK-293
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12822-1-AP targets SRRM1 in WB, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SRRM1 fusion protein Ag3513 相同性解析による交差性が予測される生物種
Full Name serine/arginine repetitive matrix 1
Calculated molecular weight 904 aa, 102 kDa
Observed molecular weight 120-160 kDa
GenBank accession numberBC036187
Gene symbol SRRM1
Gene ID (NCBI) 10250
RRIDAB_2286613
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SRRM1, also named as SRM160 and POP101, contains one PWI domain and belongs to the splicing factor SR family. As part of pre- and post-splicing multiprotein mRNP complexes, SRRM1 is involved in numerous pre-mRNA processing events. It promotes constitutive and exonic splicing enhancer (ESE)-dependent splicing activation by bridging together sequence-specific (SR family proteins, SFRS4, SFRS5 and TRA2B/SFRS10) and basal snRNP (SNRP70 and SNRPA1) factors of the spliceosome. SRRM1 stimulates mRNA 3'-end cleavage independently of the formation of an exon junction complex. It binds both pre-mRNA and spliced mRNA 20-25 nt upstream of exon-exon junctions. SRRM1 binds RNA and DNA with low sequence specificity and has similar preference for either double- or single-stranded nucleic acid substrates. SRRM1, a protein of high molecular weight(120-160kd), has a heavy glycosylation level and is normally highly phosphorylated. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human SRRM1.

Protocols

Product Specific Protocols
WB protocol for SRRM1 antibody 12822-1-APDownload protocol
IP protocol for SRRM1 antibody 12822-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Host Microbe

Quantitative phosphoproteomics reveals extensive cellular reprogramming during HIV-1 entry.

Authors - Wojcechowskyj Jason A JA
  • KD Validated
View Article
humanWB

Biochim Biophys Acta Mol Cell Res

Cleaved PGAM5 dephosphorylates nuclear serine/arginine-rich proteins during mitophagy.

Authors - Taiki Baba
View Article
humanCoIP

Tissue Cell

SRRM1 promotes the proliferation, migration, and invasion of hepatocellular carcinoma cells by regulating the JAK/STAT signaling pathway

Authors - Xiaoyan Song
View Article