Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse liver tissue, HeLa cells, Jurkat cells, UV treated HEK-293 |
| Positive IP detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| CoIP | See 1 publications below |
Product Information
12822-1-AP targets SRRM1 in WB, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SRRM1 fusion protein Ag3513 相同性解析による交差性が予測される生物種 |
| Full Name | serine/arginine repetitive matrix 1 |
| Calculated molecular weight | 904 aa, 102 kDa |
| Observed molecular weight | 120-160 kDa |
| GenBank accession number | BC036187 |
| Gene Symbol | SRRM1 |
| Gene ID (NCBI) | 10250 |
| RRID | AB_2286613 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8IYB3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SRRM1, also named as SRM160 and POP101, contains one PWI domain and belongs to the splicing factor SR family. As part of pre- and post-splicing multiprotein mRNP complexes, SRRM1 is involved in numerous pre-mRNA processing events. It promotes constitutive and exonic splicing enhancer (ESE)-dependent splicing activation by bridging together sequence-specific (SR family proteins, SFRS4, SFRS5 and TRA2B/SFRS10) and basal snRNP (SNRP70 and SNRPA1) factors of the spliceosome. SRRM1 stimulates mRNA 3'-end cleavage independently of the formation of an exon junction complex. It binds both pre-mRNA and spliced mRNA 20-25 nt upstream of exon-exon junctions. SRRM1 binds RNA and DNA with low sequence specificity and has similar preference for either double- or single-stranded nucleic acid substrates. SRRM1, a protein of high molecular weight(120-160kd), has a heavy glycosylation level and is normally highly phosphorylated. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human SRRM1.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SRRM1 antibody 12822-1-AP | Download protocol |
| IP protocol for SRRM1 antibody 12822-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Host Microbe Quantitative phosphoproteomics reveals extensive cellular reprogramming during HIV-1 entry.
| ||
Biochim Biophys Acta Mol Cell Res Cleaved PGAM5 dephosphorylates nuclear serine/arginine-rich proteins during mitophagy. | ||
Tissue Cell SRRM1 promotes the proliferation, migration, and invasion of hepatocellular carcinoma cells by regulating the JAK/STAT signaling pathway |