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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SRRT Polyclonal antibody (20353-1-AP)

    WB analysis using 20353-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20353-1-AP (SRRT antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using SRRT Polyclonal antibody (20353-1-AP)

    WB analysis of NIH/3T3 using 20353-1-AP

    WB result of SRRT antibody (20353-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SRRT transfected NIH/3T3 cells.

  • Western Blot (WB) analysis of various lysates using SRRT Polyclonal antibody (20353-1-AP)

    WB analysis using 20353-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20353-1-AP (SRRT antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Jurkat cells using SRRT Polyclonal antibody (20353-1-AP)

    IP experiment of Jurkat using 20353-1-AP

    IP result of anti-SRRT (IP:20353-1-AP, 4ug; Detection:20353-1-AP 1:30000) with Jurkat cells lysate 1440 ug.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using SRRT Polyclonal antibody (20353-1-AP)

    IF Staining of MCF-7 using 20353-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SRRT antibody (20353-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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  • Featured Product
  • KD/KO Validated

SRRT Polyclonal antibody

SRRT Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 20353-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, ELISA

Synonyms

serrate, ASR2, Arsenite-resistance protein 2, Arsenite resistance protein 2, ARS2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, NIH/3T3 cells, HepG2 cells, K-562 cells
Positive IP detected inJurkat cells
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20353-1-AP targets SRRT in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SRRT fusion protein Ag14196 相同性解析による交差性が予測される生物種
Full Name serrate RNA effector molecule homolog (Arabidopsis)
Calculated molecular weight 876 aa, 101 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC000082
Gene Symbol SRRT
Gene ID (NCBI) 51593
RRIDAB_3085603
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BXP5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SRRT (Serrate RNA effector molecule homolog) is also named as ARS2 (Arsenite-resistance protein 2). ARS2/SRRT is an essential eukaryotic protein that has emerged as a critical factor in the sorting of functional from non-functional RNA polymerase II (Pol II) transcripts (PMID: 34060620). SRRT protein is a key component of the nuclear cap-binding complex (CBC), which allows it to modulate the expression of various miRNAs. The SRRT protein is widely expressed throughout the eukaryotes, but not in budding yeast. SRRT has been shown to play a vital role in stem-cell renewal, proliferation, hematopoiesis, and RNA-mediated gene silencing and regulation of miRNA biogenesis (PMID: 37345203). SRRT is involved in cellular proliferation and colony formation (tumorigenicity). Similarly, high SRRT expression has been found in cholangiocarcinoma. ARS2 showed ubiquitous expression, with the highest levels in mouse testes, heart, and liver and human heart and skeletal muscle (PMID: 18086880).

Protocols

Product Specific Protocols
WB protocol for SRRT antibody 20353-1-APDownload protocol
IF protocol for SRRT antibody 20353-1-APDownload protocol
IP protocol for SRRT antibody 20353-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols