Validation Data Gallery

Filter: AllWBIPIHC

WB analysis using 14273-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14273-1-AP (SRX1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 14273-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 14273-1-AP (SRX1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of Transfected HEK-293 using 14273-1-AP
Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 14273-1-AP (SRX1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 14273-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 14273-1-AP (SRX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IP experiment of A549 using 14273-1-AP
IP result of anti-SRX1 (IP:14273-1-AP, 3ug; Detection:14273-1-AP 1:400) with A549 cells lysate 3300ug.
IHC staining of human lung cancer using 14273-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14273-1-AP (SRX1 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human lung cancer using 14273-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14273-1-AP (SRX1 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human kidney using 14273-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 14273-1-AP (SRX1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 14273-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 14273-1-AP (SRX1 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human breast cancer using 14273-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 14273-1-AP (SRX1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human breast cancer using 14273-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 14273-1-AP (SRX1 antibody) at dilution of 1:50 (under 40x lens).

Featured Product
KD/KO Validated

SRX1 Polyclonal antibody

SRX1 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 14273-1-AP

Publications(32)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IF, IHC, ELISA

Synonyms

C20orf139, SRX, SRX1, SRXN1, Sulfiredoxin 1, YKL086W

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (32)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A549 cells, mouse liver tissue, Transfected HEK-293 cells, HepG2 cells, U-251 cells
Positive IP detected in	A549 cells
Positive IHC detected in	human lung cancer tissue, human breast cancer tissue, human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 6 publications below
WB	See 25 publications below
IHC	See 13 publications below
IF	See 2 publications below

Product Information

14273-1-AP targets SRX1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, rat, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SRX1 fusion protein Ag5613 相同性解析による交差性が予測される生物種
Full Name	sulfiredoxin 1 homolog (S. cerevisiae)
Calculated molecular weight	14 kDa
Observed molecular weight	14 kDa
GenBank accession number	BC047707
Gene Symbol	SRXN1
Gene ID (NCBI)	140809
RRID	AB_2195030
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9BYN0
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for SRX1 antibody 14273-1-AP	Download protocol
IHC protocol for SRX1 antibody 14273-1-AP	Download protocol
IP protocol for SRX1 antibody 14273-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse		Nat Commun Loss of peroxiredoxin-2 exacerbates eccentric contraction-induced force loss in dystrophin-deficient muscle. Authors - John T Olthoff View Article
human	WB,IHC	Proc Natl Acad Sci U S A Sulfiredoxin-Peroxiredoxin IV axis promotes human lung cancer progression through modulation of specific phosphokinase signaling. Authors - Wei Qiou Q View Article
human	IHC	Cancer Res Genetic polymorphisms and protein expression of NRF2 and Sulfiredoxin predict survival outcomes in breast cancer. Authors - Hartikainen Jaana M JM View Article
human	WB	Oncogene PMID: 28504720 Authors - E-J Choi View Article
human,mouse	WB,IHC	Cancer Lett Nrf2-activated expression of sulfiredoxin contributes to urethane-induced lung tumorigenesis. Authors - Murli Mishra KD Validated View Article
mouse	WB,IHC	Carcinogenesis Tumor Promoter-induced Sulfiredoxin Is Required for Mouse Skin Tumorigenesis. Authors - Lisha Wu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 14273-1-AP

WB analysis of A549 using 14273-1-AP

WB analysis of Transfected HEK-293 using 14273-1-AP

WB analysis of mouse liver using 14273-1-AP

IP experiment of A549 using 14273-1-AP

IHC staining of human lung cancer using 14273-1-AP

IHC staining of human lung cancer using 14273-1-AP

IHC staining of human kidney using 14273-1-AP

IHC staining of human kidney using 14273-1-AP

IHC staining of human breast cancer using 14273-1-AP

IHC staining of human breast cancer using 14273-1-AP

SRX1 Polyclonal antibody

SRX1 Polyclonal Antibody for WB, IP, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Loss of peroxiredoxin-2 exacerbates eccentric contraction-induced force loss in dystrophin-deficient muscle.

Sulfiredoxin-Peroxiredoxin IV axis promotes human lung cancer progression through modulation of specific phosphokinase signaling.

Genetic polymorphisms and protein expression of NRF2 and Sulfiredoxin predict survival outcomes in breast cancer.

PMID: 28504720

Nrf2-activated expression of sulfiredoxin contributes to urethane-induced lung tumorigenesis.

Tumor Promoter-induced Sulfiredoxin Is Required for Mouse Skin Tumorigenesis.