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KD/KO Validated

SSBP1 Polyclonal antibody

Name: SSBP1 Polyclonal antibody
Brand: Proteintech
SKU: 12212-1-AP

SSBP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ChIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 12212-1-AP

Synonyms

Single-stranded DNA-binding protein, mitochondrial, PWP1-interacting protein 17, PWP1 interacting protein 17, Mt-SSB, MtSSB

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis of A549 using 12212-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 12212-1-AP (SSBP1 antibody) at dilution of 1:600 incubated at 4 degree celsius over night.
WB analysis using 12212-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12212-1-AP (SSBP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of mouse pancreas using 12212-1-AP
mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 12212-1-AP (SSBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of A375 using 12212-1-AP
A375 cells were subjected to SDS PAGE followed by western blot with 12212-1-AP (SSBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of rat pancreas using 12212-1-AP
rat pancreas tissue were subjected to SDS PAGE followed by western blot with 12212-1-AP (SSBP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of human ovary cancer using 12212-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 12212-1-AP (SSBP1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 12212-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12212-1-AP (SSBP1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of A375 using 12212-1-AP
Immunofluorescent analysis of (4% PFA) fixed A375 cells using 12212-1-AP (SSBP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Cat No. 12212-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	A549 cells, A375 cells, mouse pancreas tissue, rat pancreas tissue, Caco-2 cells
Positive IHC detected in	human stomach cancer tissue, human ovary cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A375 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 6 publications below
WB	See 24 publications below
IHC	See 3 publications below
IF	See 3 publications below
ChIP	See 1 publications below

Product Information

12212-1-AP targets SSBP1 in WB, IHC, IF/ICC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, zebrafish
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SSBP1 fusion protein Ag2860 相同性解析による交差性が予測される生物種
Full Name	single-stranded DNA binding protein 1
Calculated molecular weight	148 aa, 17 kDa
Observed molecular weight	17 kDa
GenBank accession number	BC000895
Gene symbol	SSBP1
Gene ID (NCBI)	6742
RRID	AB_2195320
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SSBP1 is a housekeeping gene involved in mitochondrial biogenesis. it binds preferentially and cooperatively to ss-DNA in the maintainance of genome stability. SSBP1 acted as a homotetramer to stabilize the displaced single strand of the normal and expanded displacement loop (D loop) during mtDNA replication, thus preventing formation of secondary single-stranded DNA structures, which could stop the gamma-DNA polymerase

Protocols

Product Specific Protocols
WB protocol for SSBP1 antibody 12212-1-AP	Download protocol
IHC protocol for SSBP1 antibody 12212-1-AP	Download protocol
IF protocol for SSBP1 antibody 12212-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
	WB	Nature Stress response silencing by an E3 ligase mutated in neurodegeneration Authors - Diane L Haakonsen View Article
human	WB	Cell MOF Acetyl Transferase Regulates Transcription and Respiration in Mitochondria. Authors - Aindrila Chatterjee View Article
human	WB	Nat Cell Biol Mitochondrial RNA granules are fluid condensates positioned by membrane dynamics. Authors - Timo Rey View Article
human	WB	Mol Cell Single-Molecule Analysis of mtDNA Replication Uncovers the Basis of the Common Deletion. Authors - Aaron F Phillips View Article
		Sci Adv The mitochondrial single-stranded DNA binding protein is essential for initiation of mtDNA replication. Authors - Min Jiang KO Validated View Article
human	IHC	Nucleic Acids Res Top3α is the replicative topoisomerase in mitochondrial DNA replication. Authors - Anu Hangas View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SSBP1 Polyclonal antibody

SSBP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis of A549 using 12212-1-AP

WB analysis using 12212-1-AP

WB analysis of mouse pancreas using 12212-1-AP

WB analysis of A375 using 12212-1-AP

WB analysis of rat pancreas using 12212-1-AP

IHC staining of human ovary cancer using 12212-1-AP

IHC staining of human stomach cancer using 12212-1-AP

IF Staining of A375 using 12212-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Stress response silencing by an E3 ligase mutated in neurodegeneration

MOF Acetyl Transferase Regulates Transcription and Respiration in Mitochondria.

Mitochondrial RNA granules are fluid condensates positioned by membrane dynamics.

Single-Molecule Analysis of mtDNA Replication Uncovers the Basis of the Common Deletion.

The mitochondrial single-stranded DNA binding protein is essential for initiation of mtDNA replication.

Top3α is the replicative topoisomerase in mitochondrial DNA replication.