Validation Data Gallery

Filter: AllWBIPIHCIF-P

WB analysis using 14157-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14157-1-AP (KRAP/SSFA2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis using 14157-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14157-1-AP (KRAP/SSFA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse testis using 14157-1-AP
IP result of anti-KRAP/SSFA2 (IP:14157-1-AP, 4ug; Detection:14157-1-AP 1:500) with mouse testis tissue lysate 4000ug.
IHC staining of human stomach using 14157-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 14157-1-AP (KRAP/SSFA2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse pancreas using 14157-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using KRAP/SSFA2 antibody (14157-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse pancreas using 14157-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using KRAP/SSFA2 antibody (14157-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

KRAP/SSFA2 Polyclonal antibody

KRAP/SSFA2 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Cat No. 14157-1-AP

Publications(8)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF-P, IP, CoIP, ELISA

Synonyms

SSFA2, ITPRID2, CS-1, CS1, CS 1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite®594

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (8)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse lung tissue, mouse testis tissue, rat lung tissue
Positive IP detected in	mouse testis tissue
Positive IHC detected in	human stomach tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse pancreas tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:200-1:800
Immunofluorescence (IF)-P	IF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 7 publications below
IHC	See 2 publications below
IF	See 3 publications below
CoIP	See 1 publications below

Product Information

14157-1-AP targets KRAP/SSFA2 in WB, IHC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, canine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag5339 Product name: Recombinant human KRAP, SSFA2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 907-1256 aa of BC028706 Sequence: SAIEMQLRRVLHDIRNSLQNLSQYPMMRGPDPAAAPYSTQKSSVLPLYENTFQELQVMRRSLNLFRTQMMDLELAMLRQQTMVYHHMTEEERFEVDQLQGLRNSVRMELQDLELQLEERLLGLEEQLRAVRMPSPFRSSALMGMCGSRSADNLSCPSPLNVMEPVTELMQEQSYLKSELGLGLGEMGFEIPPGESSESVFSQATSESSSVCSGPSHANRRTGVPSTASVGKSKTPLVARKKVFRASVALTPTAPSRTGSVQTPPDLESSEEVDAAEGAPEVVGPKSEVEEGHGKLPSMPAAEEMHKNVEQDELQQVIREVGMDPISCVILELSMICTGGGVICALEDTCC 相同性解析による交差性が予測される生物種
Full Name	sperm specific antigen 2
Calculated molecular weight	138 kDa
Observed molecular weight	180 kDa
GenBank accession number	BC028706
Gene Symbol	SSFA2
Gene ID (NCBI)	6744
RRID	AB_2195472
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P28290
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

KRAP (Ki-ras-induced actin-interacting protein), also known as SSFA2, CS1 or SPAG13, is a 1,259 amino acid cytoplasmic protein associated with filamentous-actin (F-actin). KRAP is strongly expressed in pancreas and testis. It is also present in colon cancer cell lines, and the expression is up-regulated by activated K-Ras. KRAP-deficient (KO) mice display decreased adiposity and improved glucose metabolism, implicating KRAP as a potential target for the prevention or treatment of obesity and related diseases. KRAP has also been suggested to be a potential target for cancer. This antibody recognizes endogenous KRAP, which migrates with an apparent molecular mass of 180 kDa in SDS-PAGE. (PMID: 14673706; 21501587; 21873152)

Protocols

Product Specific Protocols
IF protocol for KRAP/SSFA2 antibody 14157-1-AP	Download protocol
IHC protocol for KRAP/SSFA2 antibody 14157-1-AP	Download protocol
IP protocol for KRAP/SSFA2 antibody 14157-1-AP	Download protocol
WB protocol for KRAP/SSFA2 antibody 14157-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IHC	Nat Commun KRAP tethers IP3 receptors to actin and licenses them to evoke cytosolic Ca2+ signals. Authors - Nagendra Babu Thillaiappan KD Validated View Article
canine	WB, IF	J Cell Sci Vimentin and the K-Ras-induced actin-binding protein control inositol-(1,4,5)-trisphosphate receptor redistribution during MDCK cell differentiation. Authors - Dingli Florent F KD Validated View Article
human	WB,IHC	J Proteomics Relationship between phosphorylation of sperm-specific antigen and prognosis of lung adenocarcinoma. Authors - Akiko Okayama View Article
human	WB,IF,CoIP	Reprod Biol Endocrinol A loss-of-function variant in SSFA2 causes male infertility with globozoospermia and failed oocyte activation. Authors - Gelin Huang View Article
human	WB	Front Immunol Identification of a novel cuproptosis-related gene signature and integrative analyses in patients with lower-grade gliomas Authors - Jia-hao Bao View Article
human	WB	J Cell Sci CREB regulates the expression of type 1 inositol 1,4,5-trisphosphate receptors Authors - Vikas Arige View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 14157-1-AP

WB analysis using 14157-1-AP

IP experiment of mouse testis using 14157-1-AP

IHC staining of human stomach using 14157-1-AP

IF Staining of mouse pancreas using 14157-1-AP

IF Staining of mouse pancreas using 14157-1-AP

KRAP/SSFA2 Polyclonal antibody

KRAP/SSFA2 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

KRAP tethers IP3 receptors to actin and licenses them to evoke cytosolic Ca2+ signals.

Vimentin and the K-Ras-induced actin-binding protein control inositol-(1,4,5)-trisphosphate receptor redistribution during MDCK cell differentiation.

Relationship between phosphorylation of sperm-specific antigen and prognosis of lung adenocarcinoma.

A loss-of-function variant in SSFA2 causes male infertility with globozoospermia and failed oocyte activation.

Identification of a novel cuproptosis-related gene signature and integrative analyses in patients with lower-grade gliomas

CREB regulates the expression of type 1 inositol 1,4,5-trisphosphate receptors