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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SSH3 Polyclonal antibody

SSH3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 18324-1-AP

Synonyms

hSSH 3L, SSH 3, SSH 3L, SSH like protein 3, SSH3, SSH3L

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SSH3 Polyclonal antibody (18324-1-AP)

    WB analysis using 18324-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 18324-1-AP (SSH3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using SSH3 Polyclonal antibody (18324-1-AP)

    IP experiment of HeLa using 18324-1-AP

    IP result of anti-SSH3 (IP:18324-1-AP, 4ug; Detection:18324-1-AP 1:1000) with HeLa cells lysate 2320 ug.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SSH3 Polyclonal antibody (18324-1-AP)

    IHC staining of human colon cancer using 18324-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18324-1-AP (SSH3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SSH3 Polyclonal antibody (18324-1-AP)

    IHC staining of human colon cancer using 18324-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18324-1-AP (SSH3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SSH3 Polyclonal antibody (18324-1-AP)

    IHC staining of human breast cancer using 18324-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18324-1-AP (SSH3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using SSH3 Polyclonal antibody (18324-1-AP)

    IF Staining of A431 using 18324-1-AP

    Immunofluorescent analysis of A431 cells using 18324-1-AP (SSH3 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells, HT-29 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18324-1-AP targets SSH3 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SSH3 fusion protein Ag13174 相同性解析による交差性が予測される生物種
Full Name slingshot homolog 3 (Drosophila)
Calculated molecular weight 659 aa, 73 kDa
Observed molecular weight 90-95 kDa
GenBank accession numberBC004210
Gene symbol SSH3
Gene ID (NCBI) 54961
RRIDAB_2195490
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SSH3 antibody 18324-1-APDownload protocol
IHC protocol for SSH3 antibody 18324-1-APDownload protocol
IF protocol for SSH3 antibody 18324-1-APDownload protocol
IP protocol for SSH3 antibody 18324-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC,CoIP

Am J Cancer Res

SSH3 facilitates colorectal cancer cell invasion and metastasis by affecting signaling cascades involving LIMK1/Rac1.

Authors - Yu-Han Hu
View Article