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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SSH3 Monoclonal antibody (68583-1-Ig)

    WB analysis using 68583-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68583-1-Ig (SSH3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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SSH3 Monoclonal antibody

SSH3 Monoclonal Antibody for WB, ELISA
Cat No. 68583-1-Ig
Clone No.1A9C9

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, ELISA

Synonyms

FLJ10928, FLJ20515, hSSH 3L, SSH 3, SSH 3L, SSH like protein 3, SSH3, SSH3L

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA431 cells, A549 cells, T-47D cells, MCF-7 cells, HeLa cells, HT-29 cells, HepG2 cells, HuH-7 cells, THP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68583-1-Ig targets SSH3 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen SSH3 fusion protein Ag13139 相同性解析による交差性が予測される生物種
Full Name slingshot homolog 3 (Drosophila)
Calculated molecular weight 659 aa, 73 kDa
Observed molecular weight 90-95 kDa
GenBank accession numberBC004210
Gene Symbol SSH3
Gene ID (NCBI) 54961
RRIDAB_3085283
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8TE77
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SSH3 antibody 68583-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols