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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SSR3 Polyclonal antibody (30851-1-AP)

    WB analysis using 30851-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30851-1-AP (SSR3 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using SSR3 Polyclonal antibody (30851-1-AP)

    IF Staining of mouse brain using 30851-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using SSR3 antibody (30851-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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SSR3 Polyclonal antibody

SSR3 Polyclonal Antibody for WB, IF-P, ELISA
Cat No. 30851-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF-P, ELISA

Synonyms

TRAPG, TRAP gamma, Translocon-associated protein subunit gamma, SSR-gamma, SSR gamma

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, U-87 MG cells, mouse testis tissue, rat testis tissue
Positive IF-P detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30851-1-AP targets SSR3 in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SSR3 fusion protein Ag28130 相同性解析による交差性が予測される生物種
Full Name signal sequence receptor, gamma (translocon-associated protein gamma)
Calculated molecular weight 185 aa, 21 kDa
Observed molecular weight19 kDa
GenBank accession numberBC017203
Gene Symbol SSR3
Gene ID (NCBI) 6747
RRIDAB_3086422
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ9UNL2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Mammalian TRAP is a heterotetrameric membrane protein complex which comprised of four membrane proteins/subunits: alpha, beta, gamma, and delta. SSR3 is also known as translocon-associated protein subunit gamma (TRAPG). The signal sequence receptor (SSR) is a glycosylated endoplasmic reticulum (ER) membrane receptor associated with protein translocation across the ER membrane. SSR3 assumes a central position in the mammalian TRAP complex, binding to the ribosome on the cytosolic face of the ER membrane and coordinating the remaining TRAP subunits with the ribosome and the other translocon components.

Protocols

Product Specific Protocols
WB protocol for SSR3 antibody 30851-1-APDownload protocol
IF protocol for SSR3 antibody 30851-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols