Featured Product
KD/KO Validated

SSRP1 Polyclonal antibody

Name: SSRP1 Polyclonal antibody
Brand: Proteintech
SKU: 15696-1-AP

SSRP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 15696-1-AP

Synonyms

FACT, FACT 80 kDa subunit, FACT complex subunit SSRP1, FACT80, FACTp80, hSSRP1, SSRP1, T160

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis of HeLa using 15696-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15696-1-AP (SSRP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 15696-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 15696-1-AP (SSRP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of mouse testis using 15696-1-AP
IP result of anti-SSRP1 (IP:15696-1-AP, 4ug; Detection:15696-1-AP 1:500) with mouse testis tissue lysate 2400ug.
IHC staining of human ovary tumor using 15696-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15696-1-AP (SSRP1 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human ovary tumor using 15696-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15696-1-AP (SSRP1 antibody) at dilution of 1:100 (under 10x lens).
IF Staining of HeLa using 15696-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15696-1-AP (SSRP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 15696-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, mouse testis tissue
Positive IP detected in	mouse testis tissue
Positive IHC detected in	human ovary tumor tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 5 publications below
IHC	See 3 publications below

Product Information

15696-1-AP targets SSRP1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SSRP1 fusion protein Ag8318 相同性解析による交差性が予測される生物種
Full Name	structure specific recognition protein 1
Calculated molecular weight	81 kDa
Observed molecular weight	86 kDa
GenBank accession number	BC005116
Gene symbol	SSRP1
Gene ID (NCBI)	6749
RRID	AB_2195756
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for SSRP1 antibody 15696-1-AP	Download protocol
IHC protocol for SSRP1 antibody 15696-1-AP	Download protocol
IF protocol for SSRP1 antibody 15696-1-AP	Download protocol
IP protocol for SSRP1 antibody 15696-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Sci Transl Med Therapeutic targeting of the MYC signal by inhibition of histone chaperone FACT in neuroblastoma. Authors - Daniel R Carter View Article
human	WB	ACS Appl Mater Interfaces Injectable Bacteria-Sensitive Hydrogel Promotes Repair of Infected Fractures via Sustained Release of miRNA Antagonist. Authors - Chenyan Yu KD Validated View Article
mouse	IHC	FASEB J Chronic exercise training activates histone turnover in mouse skeletal muscle fibers. Authors - Ikumi Ohsawa View Article
human	WB,IHC	Int J Med Sci SSRP1 influences colorectal cancer cell growth and apoptosis via the AKT pathway. Authors - Qian Wang KD Validated View Article
human	WB	Cell Rep TRPS1 drives heterochromatic origin refiring and cancer genome evolution. Authors - Jianguo Yang View Article
human	WB,IHC	J Cell Mol Med SSRP1 promotes colorectal cancer progression and is negatively regulated by miR-28-5p. Authors - Wei Wu KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SSRP1 Polyclonal antibody

SSRP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of HeLa using 15696-1-AP

WB analysis of mouse testis using 15696-1-AP

IP experiment of mouse testis using 15696-1-AP

IHC staining of human ovary tumor using 15696-1-AP

IHC staining of human ovary tumor using 15696-1-AP

IF Staining of HeLa using 15696-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Therapeutic targeting of the MYC signal by inhibition of histone chaperone FACT in neuroblastoma.

Injectable Bacteria-Sensitive Hydrogel Promotes Repair of Infected Fractures via Sustained Release of miRNA Antagonist.

Chronic exercise training activates histone turnover in mouse skeletal muscle fibers.

SSRP1 influences colorectal cancer cell growth and apoptosis via the AKT pathway.

TRPS1 drives heterochromatic origin refiring and cancer genome evolution.

SSRP1 promotes colorectal cancer progression and is negatively regulated by miR-28-5p.