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  • Featured Product
  • KD/KO Validated

SSRP1 Monoclonal antibody

SSRP1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3D3H4

Cat no : 67313-1-Ig

Synonyms

SSRP1, Structure-specific recognition protein 1, T160, 3D3H4, Chromatin-specific transcription elongation factor 80 kDa subunit, Facilitates chromatin transcription complex 80 kDa subunit, Facilitates chromatin transcription complex subunit SSRP1, FACT, FACT 80 kDa subunit, FACT80, FACTp80, hSSRP1, Recombination signal sequence recognition protein 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SSRP1 Monoclonal antibody (67313-1-Ig)

    WB analysis using 67313-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67313-1-Ig (SSRP1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SSRP1 Monoclonal antibody (67313-1-Ig)

    WB analysis using 67313-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67313-1-Ig (SSRP1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-12 cells using SSRP1 Monoclonal antibody (67313-1-Ig)

    WB analysis of PC-12 using 67313-1-Ig

    PC-12 cells were subjected to SDS PAGE followed by western blot with 67313-1-Ig (SSRP1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of 4T1 cells using SSRP1 Monoclonal antibody (67313-1-Ig)

    WB analysis of 4T1 using 67313-1-Ig

    4T1 cells were subjected to SDS PAGE followed by western blot with 67313-1-Ig (SSRP1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using SSRP1 Monoclonal antibody (67313-1-Ig)

    IHC staining of human ovary tumor using 67313-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67313-1-Ig (SSRP1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SSRP1 Monoclonal antibody (67313-1-Ig)

    IHC staining of human breast cancer using 67313-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67313-1-Ig (SSRP1 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SSRP1 Monoclonal antibody (67313-1-Ig)

    IHC staining of human breast cancer using 67313-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67313-1-Ig (SSRP1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using SSRP1 Monoclonal antibody (67313-1-Ig)

    IHC staining of human ovary tumor using 67313-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67313-1-Ig (SSRP1 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SSRP1 Monoclonal antibody (67313-1-Ig)

    IF Staining of HepG2 using 67313-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 67313-1-Ig (SSRP1 antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SSRP1 Monoclonal antibody (67313-1-Ig)

    IF Staining of HepG2 using 67313-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SSRP1 antibody (67313-1-Ig, Clone: 3D3H4 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inA431 cells, 4T1 cells, NCCIT cells, PC-12 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, LNCaP cells, NIH/3T3 cells
Positive IHC detected inhuman ovary tumor tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:5000-1:20000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67313-1-Ig targets SSRP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen SSRP1 fusion protein Ag8318 相同性解析による交差性が予測される生物種
Full Name structure specific recognition protein 1
Calculated molecular weight 81 kDa
Observed molecular weight 86 kDa
GenBank accession numberBC005116
Gene symbol SSRP1
Gene ID (NCBI) 6749
RRIDAB_2882573
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SSRP1 antibody 67313-1-IgDownload protocol
IHC protocol for SSRP1 antibody 67313-1-IgDownload protocol
IF protocol for SSRP1 antibody 67313-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Biomed Pharmacother

CBL0137 activates ROS/BAX signaling to promote caspase-3/GSDME-dependent pyroptosis in ovarian cancer cells

Authors - Chang Yang
  • KD Validated
View Article