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Species Reactivity

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  • Featured Product
  • KD/KO Validated

SSSCA1 Polyclonal antibody

SSSCA1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 12273-1-AP

Synonyms

Autoantigen p27, p27, SSSCA1, ZNRD2

Validation Data Gallery

Applications:
  • WB analysis of HeLa using 12273-1-AP

    WB analysis of HeLa using 12273-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12273-1-AP (SSSCA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 12273-1-AP

    WB analysis of HeLa using 12273-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12273-1-AP (SSSCA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of NIH/3T3 using 12273-1-AP

    WB analysis of NIH/3T3 using 12273-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12273-1-AP (SSSCA1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 12273-1-AP

    WB analysis of A549 using 12273-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 12273-1-AP (SSSCA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 12273-1-AP

    IP experiment of HeLa using 12273-1-AP

    IP result of anti-SSSCA1 (IP:12273-1-AP, 4ug; Detection:12273-1-AP 1:1000) with HeLa cells lysate 2000ug.

  • IHC staining of mouse testis using 12273-1-AP

    IHC staining of mouse testis using 12273-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12273-1-AP (SSSCA1 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 12273-1-AP

    IHC staining of mouse testis using 12273-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12273-1-AP (SSSCA1 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 12273-1-AP

    IF Staining of HeLa using 12273-1-AP

    Immunofluorescent analysis of Hela cells, using SSSCA1 antibody 12273-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • FC experiment of HeLa using 12273-1-AP

    FC experiment of HeLa using 12273-1-AP

    1X10^6 HeLa cells were stained with 0.2 ug SSSCA1 antibody (12273-1-AP) and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1500 (red), or stained with 0.2 ug isotype control and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1500 (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

Tested Applications

Positive WB detected inHeLa cells, A549 cells, NIH/3T3 cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

12273-1-AP targets SSSCA1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SSSCA1 fusion protein Ag2916 相同性解析による交差性が予測される生物種
Full Name Sjogren syndrome/scleroderma autoantigen 1
Calculated molecular weight 199 aa, 21 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC014791
Gene symbol SSSCA1
Gene ID (NCBI) 10534
RRIDAB_2195902
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SSSCA1, also named as Autoantigen p27, is centromere-associated protein. It may induce anti-centromere antibodies. SSSCA1 plays a role in mitosis. It's involved in Sjogren syndrome/scleroderma. The calculated MW of SSSCA1 is 21.5 kDa (native form), with modification the MW is about23- 27 kDa.

Protocols

Product Specific Protocols
WB protocol for SSSCA1 antibody 12273-1-APDownload protocol
IHC protocol for SSSCA1 antibody 12273-1-APDownload protocol
IF protocol for SSSCA1 antibody 12273-1-APDownload protocol
IP protocol for SSSCA1 antibody 12273-1-APDownload protocol
FC protocol for SSSCA1 antibody 12273-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell

Mechanism of orphan subunit recognition during assembly quality control

Authors - Yuichi Yagita
  • KO Validated
View Article