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  • Featured Product
  • KD/KO Validated

SSSCA1 Polyclonal antibody

SSSCA1 Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 12273-1-AP

Synonyms

ZNRD2, Zinc ribbon domain-containing protein 2, Protein ZNRD2, p27, Autoantigen p27

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using SSSCA1 Polyclonal antibody (12273-1-AP)

    WB analysis of HeLa using 12273-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12273-1-AP (SSSCA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using SSSCA1 Polyclonal antibody (12273-1-AP)

    WB analysis of A549 using 12273-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 12273-1-AP (SSSCA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using SSSCA1 Polyclonal antibody (12273-1-AP)

    WB analysis of HeLa using 12273-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12273-1-AP (SSSCA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using SSSCA1 Polyclonal antibody (12273-1-AP)

    WB analysis of NIH/3T3 using 12273-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12273-1-AP (SSSCA1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using SSSCA1 Polyclonal antibody (12273-1-AP)

    IP experiment of HeLa using 12273-1-AP

    IP result of anti-SSSCA1 (IP:12273-1-AP, 4ug; Detection:12273-1-AP 1:1000) with HeLa cells lysate 2000ug.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using SSSCA1 Polyclonal antibody (12273-1-AP)

    IHC staining of mouse testis using 12273-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12273-1-AP (SSSCA1 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using SSSCA1 Polyclonal antibody (12273-1-AP)

    IHC staining of mouse testis using 12273-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12273-1-AP (SSSCA1 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of HeLa cells using SSSCA1 Polyclonal antibody (12273-1-AP)

    FC experiment of HeLa using 12273-1-AP

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug SSSCA1 Polyclonal antibody (12273-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive WB detected inHeLa cells, A549 cells, NIH/3T3 cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12273-1-AP targets SSSCA1 in WB, IHC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SSSCA1 fusion protein Ag2916 相同性解析による交差性が予測される生物種
Full Name Sjogren syndrome/scleroderma autoantigen 1
Calculated molecular weight 199 aa, 21 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC014791
Gene symbol SSSCA1
Gene ID (NCBI) 10534
RRIDAB_2195902
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SSSCA1, also named as Autoantigen p27, is centromere-associated protein. It may induce anti-centromere antibodies. SSSCA1 plays a role in mitosis. It's involved in Sjogren syndrome/scleroderma. The calculated MW of SSSCA1 is 21.5 kDa (native form), with modification the MW is about23- 27 kDa.

Protocols

Product Specific Protocols
WB protocol for SSSCA1 antibody 12273-1-APDownload protocol
IHC protocol for SSSCA1 antibody 12273-1-APDownload protocol
IP protocol for SSSCA1 antibody 12273-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell

Mechanism of orphan subunit recognition during assembly quality control

Authors - Yuichi Yagita
  • KO Validated
View Article