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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SSSCA1 Monoclonal antibody, PBS Only

SSSCA1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D2E8

Cat no : 68423-1-PBS

Synonyms

Autoantigen p27, p27, SSSCA1, ZNRD2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SSSCA1 Monoclonal antibody (68423-1-Ig)

    WB analysis using 68423-1-Ig (same clone as 68423-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68423-1-Ig (SSSCA1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68423-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SSSCA1 Monoclonal antibody (68423-1-Ig)

    IF Staining of HeLa using 68423-1-Ig (same clone as 68423-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using SSSCA1 antibody (68423-1-Ig, Clone: 1D2E8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68423-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68423-1-PBS targets SSSCA1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SSSCA1 fusion protein Ag16783 相同性解析による交差性が予測される生物種
Full Name Sjogren syndrome/scleroderma autoantigen 1
Calculated molecular weight 199 aa, 21 kDa
Observed molecular weight23 kDa
GenBank accession numberBC014791
Gene symbol SSSCA1
Gene ID (NCBI) 10534
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SSSCA1, also named as Autoantigen p27, is centromere-associated protein. It may induce anti-centromere antibodies. SSSCA1 plays a role in mitosis. It's involved in Sjogren syndrome/scleroderma. The calculated MW of SSSCA1 is 21.5 kDa (native form), with modification the MW is about 23-27 kDa.