Somatostatin (64-81aa) Polyclonal antibody

Name: Somatostatin (64-81aa) Polyclonal antibody
Brand: Proteintech
SKU: 17512-1-AP
Rating: 5.00 (1 reviews)

Somatostatin (64-81aa) Polyclonal Antibody for IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

Reviews (1)

Cat no : 17512-1-AP

Synonyms

SST, somatostatin, SST, somatostatin, SMST, Growth hormone release-inhibiting factor

Validation Data Gallery

Filter:

AllIHCIF-P

IHC staining of mouse pancreas using 17512-1-AP
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 17512-1-AP (Somatostatin (64-81aa) antibody) at dilution of 1:12800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse pancreas using 17512-1-AP
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 17512-1-AP (Somatostatin (64-81aa) antibody) at dilution of 1:12800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse pancreas using 17512-1-AP
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 17512-1-AP (Somatostatin (64-81aa) antibody) at dilution of 1:12800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse pancreas using 17512-1-AP
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 17512-1-AP (Somatostatin (64-81aa) antibody) at dilution of 1:12800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat dorsal root ganglion using 17512-1-AP
Immunohistochemical analysis of paraffin-embedded rat dorsal root ganglion tissue slide using 17512-1-AP (Somatostatin (64-81aa) antibody) at dilution of 1:12800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat dorsal root ganglion using 17512-1-AP
Immunohistochemical analysis of paraffin-embedded rat dorsal root ganglion tissue slide using 17512-1-AP (Somatostatin (64-81aa) antibody) at dilution of 1:12800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 17512-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17512-1-AP (Somatostatin-Specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 17512-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17512-1-AP (Somatostatin-Specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 17512-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 17512-1-AP (Somatostatin (64-81aa) antibody) at dilution of 1:25600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 17512-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 17512-1-AP (Somatostatin (64-81aa) antibody) at dilution of 1:25600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of human pancreas using 17512-1-AP
Immunofluorescent analysis of (4% PFA) fixed human pancreas tissue using 17512-1-AP Somatostatin-Specific antibody (green) and 66198-1-Ig Insulin antibody (red) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of human pancreas using 17512-1-AP
Immunofluorescent analysis of (4% PFA) fixed human pancreas tissue using 17512-1-AP (Somatostatin-Specific antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of human pancreas using 17512-1-AP
Immunofluorescent analysis of (4% PFA) fixed human pancreas tissue using 17512-1-AP (Somatostatin-Specific antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse pancreas using 17512-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using Somatostatin (64-81aa) antibody (17512-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse brain using 17512-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Somatostatin (64-81aa) antibody (17512-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse brain using 17512-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Somatostatin (64-81aa) antibody (17512-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Cat No. 17512-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected in	mouse pancreas tissue, human pancreas cancer tissue, mouse brain tissue, rat dorsal root ganglion tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	human pancreas tissue, mouse pancreas tissue, mouse brain tissue

Recommended dilution

Application	Dilution
Immunohistochemistry (IHC)	IHC : 1:6400-1:25600
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IF	See 3 publications below

Product Information

17512-1-AP targets Somatostatin (64-81aa) in IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	somatostatin
Calculated molecular weight	13 kDa
GenBank accession number	NM_001048
Gene symbol	Somatostatin
Gene ID (NCBI)	6750
RRID	AB_2195910
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Somatostatin is a peptide hormone that regulates the endocrine system and affects neurotransmission and cell proliferation via interaction with G protein-coupled somatostatin receptors and inhibition of the release of numerous secondary hormones. Somatostatin is a useful marker of D-cells of pancreatic islet cells. Somatostatin inhibits ins and glucagon secretion. Somatostatin has two active forms produced by alternative cleavage of a single preproprotein: one of 14 amino acids, the other of 28 amino acids. This antibody can recognize the full length and propeptide of Somatostatin.

Protocols

Product Specific Protocols
IHC protocol for Somatostatin (64-81aa) antibody 17512-1-AP	Download protocol
IF protocol for Somatostatin (64-81aa) antibody 17512-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	J Clin Endocrinol Metab β-cell dedifferentiation in HOMA-βlow and HOMA-βhigh subjects Authors - Fuyun Kang View Article
rat	IF	Aging Dis PMID: 31440379 Authors - Fei Han View Article
	IF	Cell Death Dis Inhibition of Notch activity promotes pancreatic cytokeratin 5-positive cell differentiation to beta cells and improves glucose homeostasis following acute pancreatitis. Authors - Xiaoyi Zhang View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

Reyes (Verified Customer) (04-05-2024)

Somatostatin (green) marked nicely a population of my neurons in human brain FFPE cortex.

Applications: Immunofluorescence
Primary Antibody Dilution: 1:100
Cell Tissue Type: Human Brain Cortex

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Somatostatin (64-81aa) Polyclonal antibody

Somatostatin (64-81aa) Polyclonal Antibody for IHC, IF-P, ELISA

Validation Data Gallery

IHC staining of mouse pancreas using 17512-1-AP

IHC staining of mouse pancreas using 17512-1-AP

IHC staining of mouse pancreas using 17512-1-AP

IHC staining of mouse pancreas using 17512-1-AP

IHC staining of rat dorsal root ganglion using 17512-1-AP

IHC staining of rat dorsal root ganglion using 17512-1-AP

IHC staining of mouse brain using 17512-1-AP

IHC staining of mouse brain using 17512-1-AP

IHC staining of human pancreas cancer using 17512-1-AP

IHC staining of human pancreas cancer using 17512-1-AP

IF Staining of human pancreas using 17512-1-AP

IF Staining of human pancreas using 17512-1-AP

IF Staining of human pancreas using 17512-1-AP

IF Staining of mouse pancreas using 17512-1-AP

IF Staining of mouse brain using 17512-1-AP

IF Staining of mouse brain using 17512-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

β-cell dedifferentiation in HOMA-βlow and HOMA-βhigh subjects

PMID: 31440379

Inhibition of Notch activity promotes pancreatic cytokeratin 5-positive cell differentiation to beta cells and improves glucose homeostasis following acute pancreatitis.

Reviews