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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Somatostatin Recombinant antibody

Somatostatin Recombinant Antibody for IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

240816D9

Cat no : 84351-1-RR

Synonyms

SST, somatostatin, SST, SMST, Growth hormone release-inhibiting factor, 240816D9

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using Somatostatin Recombinant antibody (84351-1-RR)

    IHC staining of mouse pancreas using 84351-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 84351-1-RR (SST-Specific antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using Somatostatin Recombinant antibody (84351-1-RR)

    IHC staining of mouse pancreas using 84351-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 84351-1-RR (SST-Specific antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse pancreas tissue using Somatostatin Recombinant antibody (84351-1-RR)

    IF Staining of mouse pancreas using 84351-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using Somatostatin antibody (84351-1-RR, Clone: 240816D9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse pancreas tissue using Somatostatin Recombinant antibody (84351-1-RR)

    IF Staining of mouse pancreas using 84351-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using Somatostatin antibody (84351-1-RR, Clone: 240816D9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive IHC detected inmouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse pancreas tissue

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:5000-1:20000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84351-1-RR targets Somatostatin in IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name somatostatin
Calculated molecular weight 13 kDa
GenBank accession numberNM_001048
Gene symbol Somatostatin
Gene ID (NCBI) 6750
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Somatostatin is a peptide hormone that regulates the endocrine system and affects neurotransmission and cell proliferation via interaction with G protein-coupled somatostatin receptors and inhibition of the release of numerous secondary hormones. Somatostatin is a useful marker of D-cells of pancreatic islet cells. Somatostatin inhibits ins and glucagon secretion. Somatostatin has two active forms produced by alternative cleavage of a single preproprotein: one of 14 amino acids, the other of 28 amino acids.

Protocols

Product Specific Protocols
IHC protocol for Somatostatin antibody 84351-1-RRDownload protocol
IF protocol for Somatostatin antibody 84351-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols