Validation Data Gallery
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Tested Applications
| Positive WB detected in | HSC-T6 cells, HeLa cells, HEK-293 cells, HepG2 cells, NIH/3T3 cells, Neuro-2a cells |
| Positive IF-P detected in | mouse kidney tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IF | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
66772-1-Ig targets SSTR5 in WB, IF-P, CoIP, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
| Tested Reactivity | Human, Mouse, Rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | SSTR5 fusion protein Ag18615 相同性解析による交差性が予測される生物種 |
| Full Name | somatostatin receptor 5 |
| Calculated molecular weight | 364 aa, 39 kDa |
| Observed molecular weight | 41 kDa |
| GenBank accession number | BC146576 |
| Gene Symbol | SSTR5 |
| Gene ID (NCBI) | 6755 |
| RRID | AB_2882118 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P35346 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Somatostatin is a widely distributed peptide with a broad range of biological actions. Two biological forms of somatostatin exist: somatostatin-14 and -28. Somatostatin receptors (SSTR1, 2A and B, 3, 4 and 5) belong to the G protein coupled receptor family and have a wide expression pattern in both normal tissues and solid tumors (PMID: 23872332). SSTR5 has greater affinity for somatostatin-28 than somatostatin-14 (PMID: 8373420).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SSTR5 antibody 66772-1-Ig | Download protocol |
| IF protocol for SSTR5 antibody 66772-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Phytomedicine Differential effect of polysaccharide and nonpolysaccharide components in Sijunzi decoction on spleen deficiency syndrome and their mechanisms. | ||
Endocr J Differential effects of β-arrestin1 and β-arrestin2 on somatostatin receptors in murine AtT-20 corticotroph tumor cells. | ||
Front Endocrinol (Lausanne) Dimerization of GPCRs: Novel insight into the role of FLNA and SSAs regulating SST2 and SST5 homo- and hetero-dimer formation | ||
Cell Death Discov Repositioning Lomitapide to block ZDHHC5-dependant palmitoylation on SSTR5 leads to anti-proliferation effect in preclinical pancreatic cancer models |