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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SSU72 Polyclonal antibody (15434-1-AP)

    WB analysis using 15434-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 15434-1-AP (SSU72 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293T cells using SSU72 Polyclonal antibody (15434-1-AP)

    WB analysis of HEK-293T using 15434-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 15434-1-AP (SSU72 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using SSU72 Polyclonal antibody (15434-1-AP)

    WB analysis of HeLa using 15434-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15434-1-AP (SSU72 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using SSU72 Polyclonal antibody (15434-1-AP)

    IHC staining of human liver using 15434-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15434-1-AP (SSU72 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using SSU72 Polyclonal antibody (15434-1-AP)

    IHC staining of human liver using 15434-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15434-1-AP (SSU72 antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using SSU72 Polyclonal antibody (15434-1-AP)

    IHC staining of human kidney using 15434-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15434-1-AP (SSU72 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using SSU72 Polyclonal antibody (15434-1-AP)

    IHC staining of human kidney using 15434-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15434-1-AP (SSU72 antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SSU72 Polyclonal antibody (15434-1-AP)

    IHC staining of human breast cancer using 15434-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15434-1-AP (SSU72 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SSU72 Polyclonal antibody (15434-1-AP)

    IHC staining of human breast cancer using 15434-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15434-1-AP (SSU72 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using SSU72 Polyclonal antibody (15434-1-AP)

    IHC staining of human prostate cancer using 15434-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15434-1-AP (SSU72 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using SSU72 Polyclonal antibody (15434-1-AP)

    IHC staining of human prostate cancer using 15434-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15434-1-AP (SSU72 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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SSU72 Polyclonal antibody

SSU72 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15434-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

RNA polymerase II subunit A C-terminal domain phosphatase SSU72, PNAS-120, PNAS 120, HSPC182, EC:3.1.3.16

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293T cells, HeLa cells
Positive IHC detected inhuman liver tissue, human breast cancer tissue, human prostate cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15434-1-AP targets SSU72 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SSU72 fusion protein Ag7699 相同性解析による交差性が予測される生物種
Full Name SSU72 RNA polymerase II CTD phosphatase homolog (S. cerevisiae)
Calculated molecular weight 23 kDa
Observed molecular weight30 kDa
GenBank accession numberBC008070
Gene Symbol SSU72
Gene ID (NCBI) 29101
RRIDAB_2878138
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NP77
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SSU72 antibody 15434-1-APDownload protocol
IHC protocol for SSU72 antibody 15434-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols