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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SSX4 Polyclonal antibody

SSX4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15876-1-AP

Synonyms

Cancer/testis antigen 5.4, CT5.4, Protein SSX4, SSX4, SSX4A, SSX4B

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using SSX4 Polyclonal antibody (15876-1-AP)

    WB analysis of MCF-7 using 15876-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 15876-1-AP (SSX4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using SSX4 Polyclonal antibody (15876-1-AP)

    WB analysis of K-562 using 15876-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 15876-1-AP (SSX4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using SSX4 Polyclonal antibody (15876-1-AP)

    IHC staining of human testis using 15876-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 15876-1-AP (SSX4 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using SSX4 Polyclonal antibody (15876-1-AP)

    IHC staining of human testis using 15876-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 15876-1-AP (SSX4 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

Positive WB detected inMCF7 cells, A549 cells, K-562 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15876-1-AP targets SSX4 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SSX4 fusion protein Ag8637 相同性解析による交差性が予測される生物種
Full Name synovial sarcoma, X breakpoint 4
Calculated molecular weight 188 aa, 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC005325
Gene symbol SSX4
Gene ID (NCBI) 6759
RRIDAB_2035018
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SSX4 antibody 15876-1-APDownload protocol
IHC protocol for SSX4 antibody 15876-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols